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Nitrogen fixation straw fermented fertile special for peanuts and method for producing same

A technology for fermenting fertilizer and nitrogen-fixing bacillus, which is applied in the field of nitrogen-fixing straw fermenting fertilizer for peanuts, and achieves the effects of making up for slow growth, strong nitrogen-fixing ability and increasing yield

Active Publication Date: 2014-01-01
深蓝柚子肥业(青岛)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the pure natural state, the number of beneficial microorganisms is not enough, and the force is also limited

Method used

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  • Nitrogen fixation straw fermented fertile special for peanuts and method for producing same
  • Nitrogen fixation straw fermented fertile special for peanuts and method for producing same
  • Nitrogen fixation straw fermented fertile special for peanuts and method for producing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The preparation method of special nitrogen-fixing stalk fermented fertilizer for peanuts comprises the following steps:

[0060] (1) Preparation of compound microbial agent:

[0061] Preparation of Bacillus nitrogen-fixing bacteria liquid: the culture method is liquid submerged fermentation, inoculate the bacteria of Bacillus nitrogen-fixing bacteria into the sterilized Erlenmeyer flask, the fermentation medium is: 0.5% starch, 0.03% yeast extract, 0.5% soybean meal, 0.2% urea, Dipotassium hydrogen phosphate 0.5%, potassium dihydrogen phosphate 0.25%, magnesium sulfate 0.1%, manganese sulfate 0.01%, pH7.0, fermentation conditions: culture temperature 37°C, stirring speed 150rpm, pH value not lower than 6.5, stirring culture After 7 hours, the live bacteria content reached 10 10 pfu / ml, to obtain the fermentation broth;

[0062] Preparation of nitrogen-fixing Bacillus megaterium bacteria liquid: the culture method is liquid submerged fermentation, inoculate the strain ...

Embodiment 2

[0088] (1) Preparation of compound microbial agent:

[0089] Preparation of Bacillus nitrogen-fixing bacteria liquid: the culture method is liquid submerged fermentation, inoculate the bacteria of Bacillus nitrogen-fixing bacteria into the sterilized Erlenmeyer flask, the fermentation medium is: 0.5% starch, 0.03% yeast extract, 0.5% soybean meal, 0.2% urea, Dipotassium hydrogen phosphate 0.5%, potassium dihydrogen phosphate 0.25%, magnesium sulfate 0.1%, manganese sulfate 0.01%, pH7.0, fermentation conditions: culture temperature 37°C, stirring speed 150rpm, pH value not lower than 6.5, stirring culture After 7 hours, the live bacteria content reached 10 10 pfu / ml, to obtain the fermentation broth;

[0090] Preparation of nitrogen-fixing Bacillus megaterium bacteria liquid: the culture method is liquid submerged fermentation, inoculate the strain of nitrogen-fixing Bacillus megaterium into a sterilized Erlenmeyer flask, the fermentation medium is: starch 0.5%, glucose 0.5%, ...

Embodiment 3

[0095] (1) Preparation of compound microbial agent:

[0096] Preparation of Bacillus nitrogen-fixing bacteria liquid: the culture method is liquid submerged fermentation, inoculate the bacteria of Bacillus nitrogen-fixing bacteria into the sterilized Erlenmeyer flask, the fermentation medium is: starch 0.1%, yeast extract 0.01%, soybean meal 0.2%, urea 0.1%, Dipotassium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, manganese sulfate 0.01%, pH7.0, fermentation conditions: culture temperature 37°C, stirring speed 150rpm, pH value not lower than 6.5, stirring culture After 7 hours, the live bacteria content reached 10 10 pfu / ml, to obtain the fermentation broth;

[0097] Preparation of nitrogen-fixing Bacillus megaterium bacteria liquid: the culture method is liquid submerged fermentation, inoculate the strain of nitrogen-fixing Bacillus megaterium into a sterilized Erlenmeyer flask, the fermentation medium is: starch 0.5%, glucose 0.5%, ye...

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Abstract

The invention belongs to the technical field of fertile, particularly relates to nitrogen fixation straw fermented fertile special for peanuts, and further relates to a method for producing the nitrogen fixation straw fermented fertile special for peanuts. The nitrogen fixation straw fermented fertile special for peanuts is characterized by comprising the following components of straws, brown sugar, a composite microbial agent, ammonium sulfate, diammonium hydrogen phosphate, potassium sulphate and monopotassium phosphate. The nitrogen fixation straw fermented fertile has the advantages of being strong in nitrogen fixation, far superior to nitrogen fixation bacillus megatherium with a single component or nitrogen fixation bacillus and overcoming the defects that the bacillus megatherium grows slowly and is insufficient in biomass, and metabolic products secreted by the fermented fertile can greatly promote growing of the bacillus megatherium; meanwhile, the growing of the bacillus megatherium further improves nitrogen fixation capability of the whole microflora.

Description

technical field [0001] The invention belongs to the technical field of fertilizers, and in particular relates to a special nitrogen-fixing straw fermented fertilizer for peanuts, and also relates to a production method of the special nitrogen-fixed straw fermented fertilizer for peanuts. Background technique [0002] Peanut is a leguminous oil crop with a seed oil content of about 50%. Peanuts can be infected by cowpea rhizobia, and about 2 / 3 of the nitrogen absorbed by plants comes from nitrogen fixation in root nodules. The yield is closely related to root development, nodule formation and nitrogen fixation conditions. According to the determination of relevant scientific research institutes, the nitrogen fixation amount of peanut nodules is equivalent to 30.5kg of ammonium sulfate or 13.3kg of urea. The rhizobia bacterial agent made from the high-efficiency nitrogen-fixing rhizobia strain is applied to agricultural production, and has the effects of good effect, low cos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00C05F11/08
Inventor 李昆仑柳金世
Owner 深蓝柚子肥业(青岛)有限公司
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