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Nannochloropsis sp mutant strain and application thereof

A quasi-Ninochlorococcus and microorganism technology, applied in the field of microorganisms, can solve the problem of not obtaining high temperature tolerance, and achieve the effects of strong high temperature tolerance, strong environmental adaptability and stable performance

Active Publication Date: 2013-12-25
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yang Guanpin et al. (Temperature stress treatment improved Nannochloropsis oculata) studied the fatty acid content changes of Nannochloropsis oculata strains under temperature stress treatment conditions, and did not obtain the strains with improved high temperature tolerance

Method used

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  • Nannochloropsis sp mutant strain and application thereof
  • Nannochloropsis sp mutant strain and application thereof
  • Nannochloropsis sp mutant strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Mutation screening, indoor culture and oil accumulation of embodiment 1ENN11-3 algal strain

[0040] Take the fresh sterile algae liquid of Nannochloropsis pseudochloropsis in the logarithmic phase, undergo mutagenesis treatment with monofunctional alkylating agent methylnitrosonitrosoguanidine (MNNG) and outdoor high temperature and pressure treatment, lay flat plates, and pick individual larger ones. A total of 30 strains of single algae colonies entered high-temperature primary screening and high-temperature secondary screening to obtain ENN11-3 algal strains.

[0041] The ENN11-3 green swimming cells in the logarithmic growth phase were inoculated in the prepared seawater medium (see Table 1 for the formula) to make the cell density reach an OD750 between 0.8-1.2, and the wild-type ENN11 was used as a control. During the cultivation process, the light intensity is controlled at 50-500umol / m 2 .s, during the culture period, adjust the pH value of the culture medium ...

Embodiment 2

[0058] The outdoor tubular high-temperature cultivation of embodiment 2ENN11-3 algae strain

[0059] Inoculate the algal species in the logarithmic growth phase in a 60*5cm tubular reactor, use seawater medium for cultivation, and use wild-type ENN11 as a control, set two parallel samples for each algae strain, and place them in an outdoor water heater Temperature-controlled natural light for high-temperature farming. The conditions during the culture period are as follows: the first 5 days of culture are at natural temperature, and the temperature range in 24 hours is mainly between 17-37°C. After cultivating to the 6th day, start to increase the temperature. The daytime temperature is mostly maintained at about 40°C, and the nighttime temperature is basically maintained above 25°C ( Figure 4 ). From 9:00 to 17:00, the range of illumination changes is mainly between 300-800umol / m2 / s on the sunny side, and basically less than 200umol / m2 / s on the backlit side. Feed a certai...

Embodiment 3

[0061] Example 3 Indoor culture and oil accumulation of ENN11-3 Nannochloropsis

[0062] Embodiment 3 The outdoor high-temperature culture of plate type of ENN11-3 algae strain

[0063] The algal species in the logarithmic growth phase were inoculated in a 50*50*5cm plate reactor, cultured with seawater medium, and wild-type ENN11 was used as a control, and placed in outdoor natural high temperature conditions in summer for growth and culture. During the culture period, the conditions As follows: the culture cycle is 11 days, the temperature range from 9:00 to 17:00 is mainly between 35-40°C, and the night temperature is mostly around 25°C ( Image 6 ). From 9:00 to 17:00, the range of light changes is mainly between 500-1000umol / m2 / s on the sunny side, and the light intensity on the backlight side is basically around 300umol / m2 / s. Feed a certain proportion of CO2 (purity above 95%), and control the pH value between 7-9. Sampling was taken intermittently during the cultivat...

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Abstract

The invention discloses Nannochloropsis sp and an application thereof. The Nannochloropsis sp disclosed by the invention has the advantages of strong environmental adaptability, strong high temperature tolerance, high content of grease, especially eicosapentaenoic acid, strong CO2 treatment capacity, and better suitability for industrial application and the like, and can be used for production of biodiesel, production of foods, health care products, feeds and baits for aquatic products and production of medicaments, as well as high-temperature farming.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a Nannochloropsis mutant strain ENN11-3 (Nannochloropsis sp) and an application thereof. Background technique [0002] With the gradual depletion of the world's fossil fuel reserves, the continuous increase of carbon dioxide emissions and the increasing warming of the global climate, human survival and development have faced unprecedented challenges. Therefore, countries all over the world are actively looking for renewable energy sources that can replace petroleum products. Among them, biodiesel is an important bioenergy source. If there are too many, the problem of "competing with people for food and land with food" will arise, which will lead to the embarrassing result of "the energy crisis has been solved, but the food crisis has emerged". [0003] Microalgae is a kind of low-level plants that are widely distributed on land and in the ocean, rich in nutrients, short in growth cycle, ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64A23L1/29A23K1/14A01K97/04A61K36/05C12P7/06C02F3/32B01D53/84B01D53/62C12R1/89A23L33/00
CPCY02A50/20Y02E50/10Y02P20/59
Inventor 李青罗少敬吴洪
Owner ENN SCI & TECH DEV
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