Method for detecting whitening efficacy of melothria heterophylla root extracts

Abstract

The invention discloses a method for detecting whitening efficacy of melothria heterophylla root extracts and relates to natural melothria heterophylla root extracts. The method comprises the following steps: (1) preparing a tyrosinase solution, an L-tyrosine solution and a PBS (phosphate buffer saline) solution as base solutions, then preparing four to-be-detected solutions, namely an arbutin solution, a supercritical CO2 extracted melothria heterophylla root extract solution, a water extracted melothria heterophylla root extract solution and an alcohol extracted melothria heterophylla root extract solution, quantitatively adding each base solution and one of the four to-be-detected solutions in four test tubes and shaking the solutions to be uniform; (2) heating the four test tubes in a water bath kettle, respectively determining the absorbance of each solution in each test tube under a wave length of 475nm and calculating the inhibition ratio of the to-be-detected solution with the concentration to tyrosinase; and (3) repeating the steps (1) and (2), determining the inhibition ratio of the other to-be-detected solutions to tyrosinase, determining the inhibition ratio of the other to-be-detected solutions with different concentrations to tyrosinase, and then according to the comparison of determination results, obtaining the whitening efficacy of each melothria heterophylla root extract and arbutin under the same concentration as well as the whitening efficacy of each melothria heterophylla root extract or arbutin under different concentrations.

Description

technical field [0001] The invention relates to a natural extract of Potassium officinalis, in particular to a method for detecting the whitening effect of the extract from Potassium officinalis. Background technique [0002] White pomegranate is a plant of the genus Vitis vinifera, also known as white root, five-clawed vine, and mountain melon, etc. It has the effects of clearing away heat and detoxifying, eliminating carbuncle and dispelling stagnation, promoting muscle growth and relieving pain. Baikang has a long history of being used as medicine, mainly using its dried root tuber, which has been recorded in "Famous Doctors" as early as, and it is an important medicine for treating furuncles by doctors of all dynasties. Modern clinical and pharmacological studies have shown that the root extract of Potassium officinalis has immune-regulating, anti-bacterial, anti-tumor and excitatory effects, and can be used to treat various acne, ulcers, burns, chapped, chloasma and age...

AI Technical Summary

Problems solved by technology

Currently commonly used anti-aging efficacy testing methods include in vivo experiment (Blumberg J.Use of Biomarkers of Oxidative Stress in Research Studies.J Nutr., 2004,134(11):3188-3189) and in vitro experiment (Liu RH, Finley J.Potential cell culture models for antioxidant research.J Agric Food Chem.,2005,53:4311–4314), but there are generally disadvantages such as long measurement period, high experimental cost, and complicated operation

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