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Method for preparing glyceride-type fish oil rich in n-3 polyunsaturated fatty acids

A technology of unsaturated fatty acid and glyceride type fish, which is applied in the field of marine biology and can solve problems such as unsmooth reactions, easy encapsulation and immobilization of enzymes, etc.

Active Publication Date: 2013-12-11
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is a novel method for preparing glyceride-type fish oil rich in n-3 polyunsaturated fatty acids, which can overcome the traditional preparation of glyceride-type fish oil rich in n-3 polyunsaturated fatty acids The method has the disadvantage that the glycerol is easy to wrap the immobilized enzyme and the reaction cannot be carried out smoothly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Weigh 20g of food grade glycerol triacetate and 100g of ethyl ester type fish oil (33% EPA content, 22% DHA content) into a stirred tank reactor connected to a condenser and a vacuum pump and preheat to 50 ° C, and then to the reaction 10 g of immobilized lipase Lipozyme 435 (provided by Novozymes) was added to the container, sealed, and stirred under the condition of 400 Pa (60 rpm) for reaction for 36 hours. After the reaction, release the reaction mixture and filter to obtain a mixture of fish oil ethyl ester and glyceride, carry out molecular distillation at 150°C and a pressure of 0.50Pa, take the heavy phase of molecular distillation and undergo decolorization, winterization, cold filtration and deodorization to obtain a clear color Lightly glyceride-type fish oil products, in which the content of n-3 polyunsaturated fatty acid triglycerides is 74.36%.

Embodiment 2

[0071] Weigh 10g food grade triacetin, 5g diacetin and 100g ethyl ester type fish oil (EPA content is 45%, DHA content is 35%), add to the stirred tank reactor connected with condenser and vacuum pump and preheat to At 60°C, 7.5 g of immobilized lipase Lipozyme 435 (provided by Novozymes) was added to the reactor, sealed, stirred at 600 Pa (60 rpm) and reacted for 72 hours. After the reaction, release the reaction mixture and filter to obtain a mixture of fish oil ethyl ester and glyceride. Molecular distillation is carried out at 160°C and an absolute pressure of 0.50Pa. The heavy phase of molecular distillation is decolorized, winterized, cold filtered and deodorized to obtain clarification. Light-colored glyceride-type fish oil products, in which the content of n-3 polyunsaturated fatty acid triglycerides is 85.03%.

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Abstract

The invention provides a method for preparing glyceride-type fish oil rich in n-3 polyunsaturated fatty acids. The method comprises the following steps: heating and stirring fish oil acetate, short-chain acid glyceride and immobilized lipase under a vacuum condition till complete reaction, and then removing unreacted fish oil acetate to obtain the glyceride-type fish oil rich in n-3 polyunsaturated fatty acids. According to the method provided by the invention, the defect that a reaction can not be smoothly carried out as glycerinum is easy to wrap immobilized enzyme in a traditional method for preparing the glyceride-type fish oil rich in n-3 polyunsaturated fatty acids can be overcome.

Description

technical field [0001] The invention relates to the technical field of marine organisms, in particular to a method for preparing glyceride-type fish oil rich in n-3 polyunsaturated fatty acids. Background technique [0002] n-3 polyunsaturated fatty acids (n-3 PUFA), represented by eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), have been proven to have important effects on human health. At present, the main source of EPA and DHA is fish oil, but the content of EPA and DHA in natural fish oil is low (mostly 14%-30%). In order to increase the content of EPA and DHA, people generally first convert natural fish oil into free fatty acids, methyl esters or ethyl esters, and then through physical and chemical methods (such as molecular distillation, urea inclusion, low temperature crystallization, chromatography and supercritical fluid technology) etc.) to increase the content of n-3 PUFA in it. Although the free n-3 PUFA is easy to be absorbed by the human body, it ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
Inventor 孙兆敏王静凤李兆杰薛勇王玉明徐杰薛长湖
Owner OCEAN UNIV OF CHINA
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