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Bradyrhizobium japonicum vitrified cryopreservation liquid and method for vitrified cryopreservation of bradyrhizobium japonicum

A technology for soybean rhizobia and vitrification cryopreservation, which is applied in microorganism-based methods, biochemical equipment and methods, and preservation of microorganisms, etc., can solve rare problems and achieve the effect of excellent preservation.

Active Publication Date: 2013-12-04
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of vitrification method is generally successful in the preservation of animal and plant cells, but it is extremely rare in the field of microbial preservation; moreover, the existing vitrification solution contains many components, most of which are composed of 5 to 6 compounds except water

Method used

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  • Bradyrhizobium japonicum vitrified cryopreservation liquid and method for vitrified cryopreservation of bradyrhizobium japonicum

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specific Embodiment approach 1

[0014] Specific embodiment one: In this embodiment, the soybean rhizobia vitrification solution is composed of 28% to 32% glycerin, 12% to 18% ethylene glycol, 12% to 18% DMSO and the rest of distilled water by mass percentage .

specific Embodiment approach 2

[0015] Specific embodiment two: In this embodiment, the soybean rhizobia vitrification solution is composed of 30% glycerol, 15% ethylene glycol, 15% DMSO and 40% distilled water by mass percentage.

specific Embodiment approach 3

[0016] Specific embodiment three: In this embodiment, the vitrification solution of soybean rhizobia is composed of 29% glycerin, 14% ethylene glycol, 14% DMSO and 43% distilled water by mass percentage.

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Abstract

The invention discloses bradyrhizobium japonicum vitrified cryopreservation liquid and a method for vitrified cryopreservation of bradyrhizobium japonicum and relates to microorganism vitrified cryopreservation liquid and a method for vitrified cryopreservation of microorganisms. The bradyrhizobium japonicum vitrified cryopreservation liquid contains ethanediol and is simple in compound composition. The bradyrhizobium japonicum vitrified cryopreservation liquid is composed of glycerinum, ethanediol, DMSO and distilled water. According to the method for vitrified cryopreservation of the bradyrhizobium japonicum, bradyrhizobium japonicum liquid is added into the bradyrhizobium japonicum vitrified cryopreservation liquid, and then the bradyrhizobium japonicum vitrified cryopreservation liquid is put into liquid nitrogen for fast vitrified cryopreservation. The bradyrhizobium japonicum vitrified cryopreservation liquid and the method for vitrified cryopreservation of the bradyrhizobium japonicum are used in the field of microorganism preservation.

Description

technical field [0001] The invention relates to a microorganism vitrification freezing storage solution and a method for vitrification freezing storage of microorganisms. Background technique [0002] Rhizobium is a class of Gram-negative rod-shaped bacteria that live in soil. Under suitable conditions, Rhizobium can infect legumes and perform symbiotic nodulation and nitrogen fixation with them. It is estimated that the nitrogen fixed by rhizobia accounts for about 65% of the total biological nitrogen fixation, which plays an extremely important role in agricultural production. Strain resources are an important part of my country's rich natural resource treasure house. With the continuous development of biological technology, the utilization of germplasm resources plays an increasingly important role in the research of life sciences. higher and higher requirements. In order to make the wild-type strains isolated from nature and the excellent strains obtained by artificial ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12R1/41
Inventor 于德水沙长青赵晓宇奚新伟戴肖东张先成王天亮姜宏宇
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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