Intragastric administration device for fish and administration method
An administration method and an intragastric administration technology are applied in the field of fish intragastric administration devices, which can solve the problems of inconvenient operation, large error, fish damage and the like, and achieve the effects of simple operation, small error and wide applicability.
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Embodiment 1
[0023] Production of gavage drug delivery device:
[0024] 1) The production method of the gavage drug delivery device is as follows, but not limited thereto: take a 12cm-long silicone tube of No. 10-16 as the injection hose, use scissors to smooth the edge of the incision at one end, and pass it through a transparent tube with a diameter of 2cm. The organic plastic circular plate is used as a baffle, so that it can slide along the injection hose, and then connected to a 10ml ~ 50ml syringe (including the injection barrel and the propulsion part), that is, the following figure 1 The fish gavage device shown: injection barrel (1), propulsion part (2), baffle (3) and injection hose (4).
[0025] 2) Examples of the manufacturing method of the gavage drug fixing device are as follows, but not limited thereto: Take a solid wood board with a length of 25cm, a width of 15cm, and a thickness of 1.5cm, and open 0.5cm in width, 1.5cm in depth, and 20cm in length on the inside. slots, t...
Embodiment 2
[0027] Take 100 red-fin snappers about 20 cm in length, and use the fish gavage drug delivery device prepared in Example 1 to carry out the gavage experiment.
[0028] 1. Feed preparation for gavage:
[0029] Dissolve the drug in water or a co-solvent and prepare a solution of the required concentration, stir and mix with the fish feed at a ratio of 1: (8-12) to make a liquid food, and let it stand in a refrigerator at 4°C for 3-6 hours.
[0030] 2. Administration by intragastric administration:
[0031] 1) Take out the prepared gavage feed 1 hour in advance. When the temperature is consistent with room temperature, place the injection hose of the gavage applicator in it, slowly pull the propulsion part, and stop when it reaches the required scale.
[0032] 2) Wrap the experimental fish with a wet towel first, place the experimental fish horizontally on one end of the wet towel so that the head of the fish is 1-2 cm above the edge of the towel, then grab the fish body...
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