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Biological coagulator, preparation method thereof and application thereof

A technology of biological solidification and processing methods, applied in the fields of application, food preparation, food science, etc., can solve problems such as difficult to realize industrialization, standardization, standardized production, difficult to control microbial content, complex microbial flora, etc., to improve edible value , Improve product quality, reduce the effect of management load

Inactive Publication Date: 2013-10-23
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the tofu made of chemical coagulants such as brine and gypsum, the sensory quality of tofu made of Physalis has the advantages of good water retention, fine texture, light taste, and slightly sweet. The microbial flora is relatively complex, the microbial content is difficult to control, the shelf life is short, and the different dominant flora will lead to different flavors of tofu, so it is difficult to achieve industrialization, standardization, and standardized production

Method used

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  • Biological coagulator, preparation method thereof and application thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0047] Example 1, preparation of Lactococcus lactis subspecies lactic acid seed solution:

[0048] The preparation of Lactococcus lactis subsp. lactis seed solution is to add 4g of glucose to 100mL of yellow pulp water, add 6mL of domesticated Lactococcus lactis subsp. 6 CFU / mL, cultured at 37°C for 24h.

Embodiment 2

[0049] Embodiment two, the acclimatization of Lactococcus lactis subsp. lactis strain, carries out following steps successively:

[0050] ① Strain activation: Activate and rejuvenate the standard strain of Lactococcus lactis subspecies lactic acid, select MRS broth medium, culture condition is 37°C, measure the number of viable bacteria after 24 hours, and achieve logarithmic growth after 4 subcultures. Bacteria count > 10 ６ CFU / mL;

[0051] ②Aclimation of Lactococcus lactis subsp. v) In the sterilized mixture of self-made medium, culture at 37°C for 24 hours; observe the growth of the strain in the 10% self-made medium, and continue to acclimatize; increase the proportion of self-made medium by 20% in turn, and finally make Lactococcus lactis Lactic acid subspecies reached the logarithmic growth phase in 24 hours in 100% self-made medium, and the number of viable bacteria was >10 ６ CFU / mL.

Embodiment 3

[0052] Embodiment three, the preparation method of biocoagulant:

[0053] (1). Weigh 40g of flour and add it to 4000mL of yellow syrup water, mix well to get flour-yellow syrup water mixture;

[0054] (2). The flour-yellow pulp water mixture was gelatinized in a water bath at 80°C for 5 minutes to obtain a gelatinized liquid;

[0055] (3). Use 0.1mol / L hydrochloric acid solution or 0.1mol / L sodium hydroxide solution to adjust the pH of the pasting solution to 6.0 to obtain a self-made medium;

[0056] (4). Sterilize the self-made medium at 121°C for 10 minutes, and cool to obtain the sterilized medium;

[0057] (5). Inoculate 360 ​​mL of Lactococcus lactis subsp. lactis seed liquid in 4000 mL of sterile medium to obtain a fermentation liquid;

[0058] (6). The fermentation broth was cultured and fermented at 35°C for 50 hours, pH=3.6, and the biological coagulant was obtained.

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Abstract

The invention discloses a biological coagulator, a preparation method thereof and an application thereof, and belongs to the technical fields of food and food processing. The biological coagulator is prepared by a step of adding flour according to a ratio into yellow serofluid that is used as a culture medium, wherein the weight ratio of the flour to the tofu yellow serofluid is 4-8:100; a step of adjusting pH of the tofu yellow serofluid to 6.0-6.4; a step of inoculating 9-15% of a lactococcus lactis stock solution; and a step of fermenting at a culture temperature of 35-40 DEG C for 50-70 h. The invention further discloses the preparation method of the biological coagulator and the application of the biological coagulator in preparing tofu. The tofu prepared by using the biological coagulator has suitable hardness, fine and smooth texture, no collapse after being cut and strong bean smell. A shelf life of the tofu at 4 DEG C is 11-12 days which is 3-4 days longer than a shelf life of a common acid-fluid tofu.

Description

technical field [0001] The invention belongs to the technical field of food and food processing, in particular to a biocoagulant and its preparation method and application. Background technique [0002] In the production process of traditional tofu, commonly used coagulants are magnesium chloride, calcium sulfate, gluconic acid-δ-lactone, and physalis. The tofu produced by magnesium chloride has poor water holding capacity and short shelf life; when calcium sulfate is used to make tofu, the solidification speed is slow and cannot be completely dissolved, which makes the tofu taste astringent and bitter; both magnesium chloride and calcium sulfate are chemical coagulants, and long-term consumption will endanger human health; Although glucose-δ-lactone is a new type of coagulant, the quality of tofu produced is also good, but the production cost is high, and the bean flavor is insufficient, slightly sour and astringent. Physalis is a natural coagulant formed by mixing and fer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/03A23L29/00
Inventor 罗爱平赵贵丽宋志敏
Owner GUIZHOU UNIV
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