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Method for scleroderma guani with aspergillus flavus preventing larvas of melanophila decastigma fabricius

A technology of swollen-legged wasps and Aspergillus flavus, which is applied in the field of swollen-legged wasps carrying aspergillus flavus to prevent and control the larvae of Aspergillus spp. Effect of increasing larval mortality

Inactive Publication Date: 2015-05-27
FOREST ECOLOGICAL RES INST XINJIANG ACADEMY OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solved the problems of difficult control, poor control effect and pesticide residues of Yangshiban Jiding

Method used

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  • Method for scleroderma guani with aspergillus flavus preventing larvas of melanophila decastigma fabricius
  • Method for scleroderma guani with aspergillus flavus preventing larvas of melanophila decastigma fabricius
  • Method for scleroderma guani with aspergillus flavus preventing larvas of melanophila decastigma fabricius

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Experimental program
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Effect test

Embodiment 1

[0025] The method of the present invention is located in the Karamay afforestation and emission reduction base in the southeast of Karamay City, Xinjiang. The geographic coordinates are: east longitude: 84°59′0.52″, north latitude 45°29′12.58″. Planting pure poplar forests with high density, 95% of the tree species are Russian poplar, mixed with other tree species such as elm, Populus euphratica, willow, etc., the distance between plants is 1m, the distance between rows is 2m, the average population density of each plant is 13-20, and the rate of insects is 75%. Confirmation, the specific steps are as follows:

[0026] a. Cultivation of entomogenic fungi: Aspergillus flavus was inoculated on a PDA plate at a temperature of 28°C for 5 days, and the purely cultured strain was inoculated on the slope of a 15mm×150mm PDA test tube at a temperature of 28°C for 9 days to produce a large amount of bacteria. sporulation;

[0027] B, Scleroderma sclerosum carrier: choose healthy and a...

Embodiment 2

[0030] The method of the present invention is located in the Karamay afforestation and emission reduction base in the southeast of Karamay City, Xinjiang. The geographical coordinates are: east longitude: 84°59′0.52″, north latitude 45°29′12.58″. Planting pure poplar forests with high density, 95% of the tree species are Russian poplar, mixed with other tree species such as elm, Populus euphratica, willow, etc., the distance between plants is 1m, the distance between rows is 2m, the average population density of each plant is 13-20, and the rate of insects is 75%. Confirmation, the specific steps are as follows:

[0031] a. Cultivation of entomogenic fungi: Aspergillus flavus was inoculated on a PDA plate at a temperature of 25°C and cultivated for 6 days. The purely cultured strain was inoculated on the slope of a 15mm×150mm PDA test tube and cultivated at a temperature of 25°C for 11 days to produce a large number of meristems spore;

[0032] B, sclerotidia carrier: choose ...

Embodiment 3

[0035] The method of the present invention is located in the Karamay afforestation and emission reduction base in the southeast of Karamay City, Xinjiang. The geographical coordinates are: east longitude: 84°59′0.52″, north latitude 45°29′12.58″. Planting pure poplar forests with high density, 95% of the tree species are Russian poplar, mixed with other tree species such as elm, Populus euphratica, willow, etc., the distance between plants is 1m, the distance between rows is 2m, the average population density of each plant is 13-20, and the rate of insects is 75%. Confirmation, the specific steps are as follows:

[0036] a, entomological fungal culture: Aspergillus flavus was inoculated on a PDA plate, cultivated at a temperature of 23°C for 7 days, inoculated the purely cultured bacterial strains on the slope of a PDA test tube of 15mm×150mm, and cultivated at a temperature of 23°C for 13 days to produce a large number of conidia;

[0037] b. Bacteroides carrying bacteria: se...

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Abstract

The invention relates to a method for scleroderma guani with aspergillus flavus preventing larvas of melanophila decastigma fabricius. According to the method, the wax insect buprestidae scleroderma guani with the aspergillus flavus are actively infected by the larvas of the melanophila decastigma fabricius, the probability of the larvas of the melanophila decastigma fabricius being infected by entomogenous fungus is increased when parasitism is conducted on the wax insect buprestidae scleroderma guani, synergism effect for preventing the larvas of the melanophila decastigma fabricius is obtained due to the synergy composition effect between the wax insect buprestidae scleroderma guani and the entomogenous fungus, and the death rate of the wax insect buprestidae scleroderma guani is increased. The method has stronger selectivity and specialization for hosts, has no toxic effect for higher plants and animals, does not pollute environment, and has the sustainable control effect. The method for the scleroderma guani with the aspergillus flavus preventing the larvas of the melanophila decastigma fabricius is reasonable, and good in operability, solves the problems that the prevention for the melanophila decastigma fabricius is difficult, control effect is poor and pesticide is reserved, and provides a new way for preventing the larvas of the melanophila decastigma fabricius.

Description

technical field [0001] The present invention relates to a kind of poplar ten spot jellyfish ( Melanophila picta Pallas) larval control methods, specifically relating to Scleroderma aureus Sclerodermus pupariae Aspergillus flavus Aspergillus flavus A method for controlling the larvae of the poplar ten-spotted jellyfish. Background technique [0002] The early instar larvae of Populus chinensis do damage between bark and xylem, feed on the phloem tissue of trees, and gradually penetrate into the cambium as the age of the larvae grows, and the insect feces are not discharged outside, causing the death of poplars, willows and other damaged trees. Because of the characteristics of concealed life, it is difficult to achieve a good control effect with conventional control methods. When the damage is serious, the population density of each plant can reach 50-60, or even up to 100, and the rate of damaged plants can reach 60-90%. The death of the entire forest has destroyed the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A01M99/00
Inventor 岳朝阳张新平阿里木·买买提克热曼·赛米陈晶晶张静文刘爱华焦淑萍
Owner FOREST ECOLOGICAL RES INST XINJIANG ACADEMY OF FORESTRY
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