RT-LAMP nucleic acid test strip kit for detection of porcine reproductive and respiratory syndrome virus, and applications

Abstract

The invention discloses a RT-LAMP nucleic acid test strip kit for detection of a porcine reproductive and respiratory syndrome virus, and applications The kit comprises a primer combination with a nucleotide sequence represented by SEQ ID NO. 1-6 and nucleic acid detection test strips. The usage of the kit is as follows: first preparing RT-LAMP reaction system comprising an AMV retrovirus, a 1* reaction buffer, a Bst DNA polymerase, a dNTP mixture, betaine, MgSO4, a FIP primer, a BIP primer, a LoopF primer, a LoopB primer, a F3 primer, a B3 primer, and RNA of a sample to be measured; carrying out a reaction at a constant temperature, after testing the obtained products by using the nucleic-acid-detecting test strip, judging and reading directly: the positive result is that two red strips appear, and one strip is in the detection zone while the other strip is in the control zone. The kit has the advantages of simple operation, low cost, easy observation of the reaction result, good specificity, easy popularization and application in large scope and being extremely suitable for export quarantine, food hygiene and on-site detection in animal husbandry.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an RT-LAMP nucleic acid test strip kit for detecting porcine reproductive and respiratory syndrome virus and its application. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS), commonly known as "PRRS", is caused by porcine reproductive and respiratory syndrome virus (Porcine reproductive and respiratory syndrome virus, PRRSV). A viral infectious disease characterized by reproductive failure, respiratory symptoms in piglets, and high mortality in weaned piglets. In addition, it is also clinically manifested in symptoms such as atypical estrus and low pregnancy rate in some recovered sows. The disease first broke out in the United States in 1987, and then spread rapidly around the world, causing huge economic losses to the pig industry. The International Organization for Epizootics defines it as a Class B infectious disease, and my country classifies...

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Problems solved by technology

However, this method is not accurate enough to distinguish weak positive results, and it will make it difficult to judge the experimental results because of the concentration ratio of calcein and manganese ions

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