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Production process for extracting four enzymes from cattle or pig pancreas

A production process and technology for porcine pancreas, applied in the field of bioengineering, can solve the problem of low comprehensive utilization rate of pancreas, and achieve the effects of high comprehensive utilization rate of pancreas, high activity and recovery rate, and significant economic benefits

Active Publication Date: 2013-09-04
赤峰市云淞科技发展有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented method uses an improved technique for producing various types of protein called histidine lyases (HDL). These techniques involve separating specific components such as these enzymatic reactions into different fractions that improve their overall effectiveness while minimizing disruption during processing steps like extraction. By combining this with other methods like chemical synthesis, they allow us to produce more effective drugs without compromising on quality control standards.

Problems solved by technology

Technologies aim at developing an improved way to isolate or produce specific types of pepper protein called pigment epitheliome (PE) by separating different components like lyserine, trypticulose, lysoscopyoligosaccharide, beta galactan polyglycerols, amylopectins, fibrillarin, mannitrogen monoxydimethyl hydrazinohexadecanoides, tannins, flavonoids, ellagitannanes, quercetones, caffeinamines, hydroxypyrrolone acids, oxazolidinediones, thrombocyte stimulating agents, cytokines, growth factors, antibiosis compounds, cellular signal transducers, neurotransmitters, hormonal regulators, histochemically derived nucleotides, nucleic acid bases, glutathione permeability enhancement reagent, iron salt depletion agent, nitrite decomposer, sulfurized oxygen bleach, sodium bichloroquoruminate solution, and crystal precipitation techniques with limited effectiveness on multiple enzymatic products produced during extraction due to its complexity.

Method used

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  • Production process for extracting four enzymes from cattle or pig pancreas

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Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: a kind of production technique that extracts four kinds of enzymes from bovine pancreas, it comprises the steps:

[0030] (1) Tissue mincing: remove the fat and connective tissue from the bovine pancreas, wash it with water, and mince it with a meat grinder;

[0031] (2) Acid leaching extraction: add water and sulfuric acid solution to the minced pancreas, the solid-to-liquid weight ratio is 1:4, the pH value is 2.6, stir and extract at room temperature for 15 hours to obtain an extraction mixture;

[0032] (3) Filtration and separation: first filter the extraction mixture with a drum sieve, then add perlite with 17% of the weight of bovine pancreas and stir, then use a plate and frame filter to filter and separate, collect the extraction filtrate, and discard the solid waste residue;

[0033] (4) ultrafiltration concentration: the extraction filtrate is filtered with a two-stage ultrafiltration membrane, and the ultrafiltration membrane with a molecular w...

Embodiment 2

[0043] Embodiment 2: a kind of production technique that extracts four kinds of enzymes from bovine pancreas, it comprises the steps:

[0044] (1) Tissue mincing: remove the fat and connective tissue from the bovine pancreas, wash it with water, and mince it with a meat grinder;

[0045] (2) Acid leaching extraction: add water and sulfuric acid solution to the minced pancreas, the solid-to-liquid weight ratio is 1:3, the pH value is 2.8, stir and extract at room temperature for 14 hours to obtain an extraction mixture;

[0046] (3) Filtration and separation: first filter the extraction mixture with a drum sieve, then add perlite with 16% bovine pancreas weight and stir, then use a plate and frame filter to filter and separate, collect the extraction filtrate, and discard the solid waste residue;

[0047] (4) ultrafiltration concentration: the extraction filtrate is filtered with a two-stage ultrafiltration membrane, and the ultrafiltration membrane with a molecular weight of 2...

Embodiment 3

[0057] Embodiment 3: a kind of production technique that extracts four kinds of enzymes from pig pancreas, it comprises the steps:

[0058] (1) Minced tissue: Remove fat and connective tissue from pig pancreas, wash it with water, and mince it with a meat grinder;

[0059] (2) Acid leaching extraction: add water and sulfuric acid solution to the minced pancreas, the solid-to-liquid weight ratio is 1:5, the pH value is 2.4, stir and extract at room temperature for 16 hours to obtain an extraction mixture;

[0060] (3) Filtration and separation: firstly, the leaching mixed solution is filtered with a drum sieve, then added with 18% perlite of porcine pancreas weight and stirred, and then filtered and separated by a plate-and-frame filter, the extraction filtrate is collected, and the solid waste residue is discarded;

[0061] (4) ultrafiltration concentration: the extraction filtrate is filtered with a two-stage ultrafiltration membrane, and the ultrafiltration membrane with a m...

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Abstract

The invention discloses a production process for extracting four enzymes from cattle or pig pancreas, which comprises the following steps: tissue mincing, acid leaching and extracting, filtering and separation, ultrafiltration and concentration, one-phase salting out, two-phase salting out, dissolving and activating, purifying, filtering, desalination concentration, one-phase chromatography, two-phase chromatography and the like, and is technically characterized in that segmental salting-out and segmental chromatography technologies are adopted to obtain deoxyribonuclease, chymotrypsin, trypsin and kininogenase. The production process has the advantages of mature process, simplicity and convenience in operation, low cost, and high activity and recovery rate.

Description

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Claims

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Application Information

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Owner 赤峰市云淞科技发展有限责任公司
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