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Method for stimulating rapid proliferation of CIK (Cytokine-induced Killer) cells by using DC (Dendritic Cells)

A cell stimulation and cell technology, applied in animal cells, vertebrate cells, blood/immune system cells, etc., can solve the problems of short survival period, long CIK cell expansion and culture time, slow DC cell maturation, etc., and achieve the killing function. The effect of enhancing, shortening the amplification time, and increasing the multiplication factor

Inactive Publication Date: 2013-08-21
爱瑞康医疗投资管理(北京)有限公司
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Problems solved by technology

[0011] The purpose of the embodiments of the present invention is to provide a method for using DC cells to stimulate the rapid proliferation of CIK cells, aiming to solve the problems of slow maturation of DC cells, short survival period, and long expansion and culture time of CIK cells

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  • Method for stimulating rapid proliferation of CIK (Cytokine-induced Killer) cells by using DC (Dendritic Cells)
  • Method for stimulating rapid proliferation of CIK (Cytokine-induced Killer) cells by using DC (Dendritic Cells)
  • Method for stimulating rapid proliferation of CIK (Cytokine-induced Killer) cells by using DC (Dendritic Cells)

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Embodiment Construction

[0048] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0049] figure 1 The flow chart of the method for using DC cells to stimulate rapid proliferation of CIK cells provided by the present invention is shown. For ease of illustration, only the parts relevant to the present invention are shown.

[0050] Such as figure 1 As shown, the method for using DC cells to stimulate the rapid proliferation of CIK cells provided by the embodiments of the present invention includes the following steps:

[0051] Isolation of PBMC from peripheral blood mononuclear cells;

[0052] Induction culture of cytokine-induced killer cells;

[0053] CIK cell induction culture;

[0054] DC cell ind...

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Abstract

The invention discloses a method for stimulating the rapid proliferation of CIK (Cytokine-induced Killer) cells by using DC (Dendritic Cells). The method comprises the following steps: separating peripheral blood monouclear cells (PBMC); performing induction culture on cytokine-induced killer cells, i.e., performing induction culture on the CIK cells; performing induction culture on the DC; and detecting the phenotype CD3<+>CD56<+> of the CIK cells. According to the method, the numbers of the CIK cells are amplified substantially within a short period while the CD3<+>CD56<+> ratio of the surfaces of the CIK cells is not changed sharply, so that the amplification time of the CIK is shortened obviously; and the proliferation rate is improved at the same time. Therefore, the important scientific research value is obtained.

Description

technical field [0001] The invention belongs to the field of cell culture in adoptive immunotherapy, and in particular relates to a method for using DC cells to stimulate rapid proliferation of CIK cells. Background technique [0002] One person is diagnosed with cancer every 6 minutes across the country, 8,550 people become cancer patients every day, and one person dies of cancer every seven to eight people. In the next 10 years, the incidence and mortality of cancer in China will continue to rise. From "cancer county" to "cancer village", behind the historical and geographical coordinates of cancer incidence in China is the high incidence of cancer brought about by decades of social development and lifestyle changes. Tumor is one of the major diseases threatening human health. [0003] At present, malignant tumors are the second deadliest disease in the world and the first cause of death among urban and rural residents in China. The total number of new cancer patients in...

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Application Information

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IPC IPC(8): C12N5/0783
Inventor 陈晚华孙庆文曲平波王延春颜廷骥张俊艺
Owner 爱瑞康医疗投资管理(北京)有限公司
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