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Mushroom liquid strain as well as preparation method, preparation device and application thereof

A liquid strain and preparation device technology, which is applied in the direction of botany equipment and methods, application, fertilization device, etc., can solve the problems of complex structure of liquid strain fermentation tank, unstable pH value, poor stirring effect, etc., and achieve good stirring effect, effect, effect with simple structure

Active Publication Date: 2014-08-13
WEIHAI XINBAO FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The present invention aims to solve the technical problems of complex operation, unstable pH value, long cycle, seriously low success rate and complex structure, high cost and poor stirring effect of the existing liquid strain fermentation tank. , providing a liquid strain of shiitake mushrooms with simple operation, stable pH value, short period, high success rate, simple structure, low cost, and good stirring effect, as well as its preparation method, preparation device and application

Method used

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  • Mushroom liquid strain as well as preparation method, preparation device and application thereof
  • Mushroom liquid strain as well as preparation method, preparation device and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0045] The components of the medium by weight percentage are as follows: peanut cake powder: 0.3%, brown sugar 1%, corn flour: 0.8%, peptone 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.03%, edible oil: 0.1 %, the balance is water.

[0046] Preparation of the mother seed in shake flask: Make 2500ml of medium according to the recipe, add it to a Erlenmeyer flask with a capacity of 5000ml, insert a mother seed in a test tube, keep the temperature at 26°C, and cultivate it with magnetic stirring. It can be used within 5-6 days.

[0047]Preparation of the seed tank: use a 60L tank as the seed tank, prepare 50L medium according to the requirements, cool to 26°C after sterilization, insert a bottle of the above-mentioned shaker flask, inject sterile air, and cultivate at a constant temperature of 26°C. After 5 days, the concentration of bacteria balls can reach 80%-100%. The color of bacteria balls is white and uniform in size. Sampling test shows that the pH value...

Embodiment 2

[0059] Peanut cake flour: 0.4%, brown sugar 1.5%, corn flour: 0.7%, peptone 0.15%, potassium dihydrogen phosphate 0.13%, magnesium sulfate: 0.04%, edible oil: 0.15%, and the balance is water.

[0060] Step is with embodiment 1, and concrete condition is as follows:

[0061] The temperature in step (1) is 70°C; the temperature in step (6) is 123°C; the time is 1.3 hours; in step (7), cool to 25°C; keep the temperature at 25°C; cultivate for 5 days.

Embodiment 3

[0063] Peanut cake powder: 0.5%, brown sugar 2%, corn flour: 0.55%, peptone 0.2%, potassium dihydrogen phosphate 0.15%, magnesium sulfate: 0.05%, edible oil: 0.2%, and the balance is water.

[0064] Step is with embodiment 1, and concrete condition is as follows:

[0065] The temperature in step (1) is 70°C; the temperature in step (6) is 125°C; the time is 1 hour; in step (7), cool to 28°C; keep the temperature at 28°C; cultivate for 4 days.

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Abstract

The invention provides a mushroom liquid strain as well as a preparation method, a preparation device and application thereof. The mushroom liquid strain contains culture medium, wherein the culture medium comprises the component by weight percent: peanut meal, brown sugar, corn meal, peptone, potassium dihydrogen phosphate, magnesium sulfate, edible oil and the balance of water. The mushroom liquid strain as well as the preparation method, the preparation device and the application thereof can be widely used in the field of mushroom cultivation and planting.

Description

technical field [0001] The invention relates to a strain and its preparation method, preparation device and application, in particular to a mushroom liquid strain and its preparation method, preparation device and application. Background technique [0002] Lentinus edodes is rich in nutrition, unique in fragrance, and has anti-cancer and anti-cancer effects. It is a famous edible fungus with a long history and is favored by consumers at home and abroad. [0003] As far as the production status of shiitake mushrooms is concerned, more than 90% of practitioners continue to use the long-standing solid strain inoculation technique, while liquid strains, a new technology with low cost, convenient operation, rapid germination, and high biotransformation rate, are widely used in the industry. The field of shiitake mushroom cultivation is rarely mentioned. [0004] The Chinese invention patent application with the application number CN02124094.9 discloses a liquid strain of shiitak...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G1/00A01G1/04
Inventor 汪建党侯艳平
Owner WEIHAI XINBAO FOOD
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