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Gene sequence for synthesizing relative transcription factor SmMYB by eggplant anthocyanidin

A technology of gene sequence and transcription factor, which is applied in the field of gene sequence of eggplant anthocyanin synthesis-related transcription factor SmMYB, can solve the problem that the same or similar technical subject has not been found, and achieve high anthocyanin content and resistance quality Effect

Inactive Publication Date: 2013-07-24
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After searching the prior art literature, no technical subject identical or similar to the present invention has been found

Method used

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  • Gene sequence for synthesizing relative transcription factor SmMYB by eggplant anthocyanidin
  • Gene sequence for synthesizing relative transcription factor SmMYB by eggplant anthocyanidin
  • Gene sequence for synthesizing relative transcription factor SmMYB by eggplant anthocyanidin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1, the cloning and tissue expression characteristics analysis of the transcription factor SmMYB gene related to eggplant anthocyanin synthesis.

[0049] Step 1, cloning the transcription factor SmMYB gene related to eggplant anthocyanin synthesis.

[0050] 1. Experimental material selection——

[0051] The excellent eggplant germplasm resources YZ14 and YZ3 preserved by the applicant were used as experimental materials. The phenotype of YZ14 is spineless, purple stem, purple flower, and purple fruit, hereinafter referred to as purple eggplant; the phenotype of YZ3 is thorny, green stem, white flower, and ginkgo fruit, with obvious wild eggplant traits, hereinafter referred to as white eggplant . The experimental materials were all planted in artificial plastic greenhouses at the Aerospace Breeding Base in Pujiang Town, Minhang District, Shanghai, where seedlings were raised, grown and fruited under natural conditions. Get the root, purple stem, purple leaf, pu...

Embodiment 2

[0076] Example 2, after shading treatment, the anthocyanin content and SmMYB expression in the pericarp of purple eggplant.

[0077] 1. Experimental design of bagging shading treatment for purple eggplant.

[0078] It takes about 20 days for purple eggplant YZ14 from flowering stage to commercial maturity stage. Flowers with petals open at 90° are selected for bagging and shading treatment. The treatments were divided into A, B, C, D, E, F, the negative control CK- and the positive control CK+ (Table 2). Fruit harvesting was carried out, and eggplant peels were peeled off as experimental materials.

[0079] 2. Determination of anthocyanin content.

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Abstract

The invention discloses a gene sequence for synthesizing a relative transcription factor SmMYB by eggplant anthocyanidin, comprising a nucleotide sequence shown in 1st-1035th nucleotides in SEQ ID NO.1, wherein a polypeptide coded by the gene sequence comprises an amino acid sequence is shown in SEQ ID NO.2. The gene sequence in the invention is a key regulation and control gene for synthesizing eggplant anthocyanidin, the relative transcription factor SmMYB is synthesized by eggplant anthocyanidin. A downstream gene which is regulated and controlled by the gene sequence and is closely related to anthocyanidin synthesis, and other transcription factor genes manually acting with the gene sequence, are screened. The gene sequence in the invention is applied to obtain an eggplant kind with high anthocyanidin content and excellent resistance quality.

Description

technical field [0001] The present invention relates to a gene sequence in the field of biotechnology, in particular to a gene sequence of a transcription factor SmMYB related to eggplant anthocyanin synthesis. Background technique [0002] Anthocyanins are widely found in nature and belong to flavonoids. They usually form glycosides with sugars such as glucose, called anthocyanins. Because anthocyanins have biochemical and pharmacological effects such as anti-oxidation and free radical scavenging, since the 1980s, research on it has become increasingly extensive and in-depth around the world. Eggplant is a vegetable crop that originated in Asia and is widely grown around the world. Different varieties vary greatly in shape and color, and purple eggplant is the most common variety in the market. The peel of purple eggplant is rich in delphinidin-3-rutinoside, which is a highly stable anthocyanin with rich nutritional value. Nowadays, improving flavonoids (anthocyanins) in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415A01H5/00
Inventor 陈火英邵文婷韩洪强葛海燕
Owner SHANGHAI JIAO TONG UNIV
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