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Application of SKF96365 in treatment of cerebral angiospasm after subarachnoid hemorrhage

A technique for cerebral vasospasm and subarachnoid space, applied in the direction of cardiovascular system diseases, drug combination, etc., to achieve the effect of alleviating lumen stenosis and vasospasm

Inactive Publication Date: 2013-07-24
THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there are no studies on the role of TRPC1 and TRPC4 in the development of CVS after SAH

Method used

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  • Application of SKF96365 in treatment of cerebral angiospasm after subarachnoid hemorrhage
  • Application of SKF96365 in treatment of cerebral angiospasm after subarachnoid hemorrhage
  • Application of SKF96365 in treatment of cerebral angiospasm after subarachnoid hemorrhage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Changes in expression of TRPC1 and TRPC4 in the basilar artery wall after subarachnoid hemorrhage in rats

[0042] a material

[0043] 1 Main reagents and medicines

[0044] 1.1 Conventional reagents

[0045] 10% chloral hydrate, preparation room of the First Affiliated Hospital of Soochow University; ready-to-use SABC kit, Wuhan Boster Company; DAB chromogenic kit, Wuhan Boster Company. The preparation method of 4% paraformaldehyde is as follows: Add 4 grams of paraformaldehyde to 0.1mol / L PBS100ml, heat to 60°C and stir to dissolve, filter and store at 4°C.

[0046] The preparation method of 0.1mol / L PBS buffer solution (pH7.4) is as follows: KH 2 PO 4 13.60g, NaOH 3.20g, add double distilled water to 1000ml, adjust the pH value to 7.4 with hydrochloric acid and sodium hydroxide.

[0047] RIPA lysate, Beijing Presley Biological Company; phenylmethylsulfonyl fluoride (PMSF), Beijing Presley Biological Company; protein quantification kit (BCA method), Bei...

Embodiment 2

[0181] Example 2 Experiment of the intervention effect of TRPC1 and TRPC4 inhibitors on cerebral vasospasm after subarachnoid hemorrhage in rats

[0182] In this example, the SAH model of rats with secondary blood injection into the cistern magna was used. By observing the morphology of the rat basilar artery, and using the Western blot method to observe the effects of TRPC1 and TRPC4 inhibitors on CVS after SAH, to explore the inhibition of TRPC1 and TRPC4 Whether the drug has an intervening effect on the CVS of rats after SAH, so as to provide an experimental basis for the prevention and treatment of cerebral vasospasm after SAH.

[0183] 1. Materials

[0184] 1.1 Reagents and experimental equipment

[0185] Except for SKF96365, the main medicines and reagents were purchased from Sigma Company of the United States; see Example 1 for other reagents and medicines. Refer to Example 1 for main instruments.

[0186] 1.2 Experimental animals

[0187] Adult healthy male SD rats...

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Abstract

The invention discloses application of SKF96365 in preparation of a medical composition for treating cerebral angiospasm. According to the invention, a cisterna magna primary blood injection method rat SAH model is adopted to disclose TRPC (Transient Receptor Potential Canonical)1 and TRPC4 expression up-regulation of vascular wall after SAH. The activating level is closely associated with the pathologic change degree of spasm vascular wall in expression time phase, so as to indicate that TRPC1 and TRPC4 participate in occurrence and development of CVS (Cerebral Vascular Spasm). A TRPC channel paralyser SKF96365 is used to prevent and treat cerebral vascular spasm, and the TRPC channel is paralyzed to limit calcium influx of smooth muscle cell, so that the degree of lumen stenosis and lumen thickening can be partially relieved, and a certain treating effect is shown.

Description

technical field [0001] The invention belongs to the technical field of new application of medicine, and specifically relates to the application of SKF96365 in treating cerebral vasospasm after subarachnoid hemorrhage. Background technique [0002] Subarachnoid hemorrhage (SAH) is a syndrome caused by blood entering the subarachnoid space in the intracranial or spinal canal due to various reasons. It is generally divided into traumatic SAH and spontaneous SAH. Caused by brain damage, which can have a variety of causes. Spontaneous SAH is a common emergency and critical disease of the nervous system, with an annual incidence of about 6-16 / 100,000 people, accounting for about 6-8% of the total incidence of stroke. Intracranial aneurysm rupture is the main cause of spontaneous SAH After hemorrhage, 30% to 90% of patients may experience different degrees of cerebral vasospasm (CVS), and some severe cases may cause local brain tissue ischemia or delayed ischemic brain damage, or ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4174A61P9/10
Inventor 王中陈罡孙晓欧
Owner THE FIRST AFFILIATED HOSPITAL OF SOOCHOW UNIV
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