A kind of inactivated lactic acid bacteria effervescent tablet and preparation method thereof
A technology for live lactic acid bacteria and effervescent tablets, applied in the field of inactivated lactic acid bacteria effervescent tablets and preparation thereof, can solve the problems of difficult control of hygienic indicators, post-fermentation of products, large consumption of cans, packaging bottles, etc., and achieves a simple and feasible preparation method. , Not easy to deteriorate, the effect of prolonging the shelf life
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Embodiment 1
[0052] The preparation of embodiment 1 inactivated lactic acid bacteria powder
[0053] S01. Inoculate Lactobacillus acidophilus (purchased from China Industrial Microorganism Culture Collection and Management Center, and those skilled in the art can also use related bacteria from other sources) according to the inoculum size of 5-10% in the conventional MRS liquid medium Cultivate at 37°C, when the number of lactic acid bacteria reaches 10 9 / mL, the culture was stopped to obtain the fermentation broth;
[0054] S02. Add glycerin or sodium alginate with a mass fraction of 0.1 to 0.5% to the fermentation broth obtained in S01 to obtain a mixed system;
[0055] S03. Heat inactivate the mixed system obtained from S02 at 60°C for 30 minutes, centrifuge at 3000-5000rmp for 5-10 minutes to collect lactic acid bacteria cells;
[0056] S04. Add soluble starch of equal quality to the thallus obtained in S03, carry out vacuum drying (60°C, 0.04MPa) to prepare inactivated lactic acid ...
Embodiment 2
[0057] The preparation of embodiment 2 inactivated lactic acid bacteria powder
[0058] S01. Inoculate Lactobacillus bulgaricus and Streptococcus thermophilus (purchased from China Industrial Microbiology Culture Collection Management Center) into milk liquid culture medium at 37°C according to the inoculation amount of 5-10%, and cultivate them when the number of lactic acid bacteria reaches 10 8-9 / mL, the culture was stopped to obtain a fermented liquid; the composition of the milk liquid medium was: 8% skimmed milk powder, 2% sucrose, and 90% water in terms of mass percentage.
[0059] S02. Add glycerin or sodium alginate with a mass fraction of 0.1 to 0.5% to the fermentation broth obtained in S01 to obtain a mixed system;
[0060] S03. Heat inactivate the mixed system obtained from S02 at 80°C for 20 minutes, centrifuge at 3000-5000rmp for 5-10 minutes to collect lactic acid bacteria cells;
[0061] S04. Add glucose of equal quality to the thallus obtained in S03, carry...
Embodiment 3
[0062] The preparation of embodiment 3 inactivated lactic acid bacteria powder
[0063] S01. Inoculate Bifidobacteria (purchased from China Industrial Microorganism Culture Collection Management Center) into conventional PYG liquid medium according to 5-10% inoculum size for anaerobic culture at 37°C. When the number of lactic acid bacteria reaches 10 7-8 / mL, the culture was stopped to obtain the fermentation broth;
[0064] S02. Adding glycerol and sodium alginate with a mass fraction of 0.1 to 0.5% in the fermentation broth obtained in S01 to obtain a mixed system;
[0065] S03. heat-inactivate the mixed system obtained in S02 at 60-80°C for 30-60 minutes, and centrifuge at 3000-5000rmp to collect lactic acid bacteria cells;
[0066] S04. Add glucose of equal quality to the thalline obtained in S03, spray dry (inlet temperature 150°C, outlet temperature 105°C) to prepare inactivated lactic acid bacteria powder, and the number of bacteria in the powder reaches 10 9 piece / g...
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