Dendrobium officinale sprout rapid propagation method with high efficiency

Abstract

The invention discloses a dendrobium officinale sprout rapid propagation method with high efficiency. The method comprises the following steps: a) selecting dendrobium officinale shoot tip or aseptic seedling as explants, performing induction and propagation culture of protocorm like bodies; b) performing differentiation and seeding formation on protocorm like bodies; c) culturing strong plantlet and rootage of seedling grown thickly; and d) hardening seedling and transplanting of the aseptic seedling. According to the invention, shoot tip and aseptic seedling are taken as the explants, thereby the problem that stem bud and top bud as the explants to induce the protocorm like bodies, the explant source is limited can be solved, the mature protocorm like bodies can be rapidly and continuously obtained with large amount, the incidence rate of the protocorm like bodies can reach as high as 99%, the seedling differentiation coefficient can reach as high as 50 strains / 0.1g protocorm like bodies, the period for acquiring the seedling is 4 months, the seedling is tidy, the rooting rate is 100%, the root length is about 5cm, and the root number can reach 10 strips / crowd, the transplanting survival rate can reach as high as more than 95%, and the purposes of shortening the seedling period, increasing the propagation coefficient and unlimited germplasm source can be reached. The rapid propagation method can rapidly produce large batch of sprouts, and is suitable for large scale production.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and specifically relates to a method for high-efficiency and rapid propagation of dendrobium candidum seedlings. The method uses plant tissue culture technology to perform efficient and rapid propagation of dendrobium candidum seedlings. Background technique [0002] Dendrobium officinale, commonly known as "gold in medicine", is an epiphytic and perennial herb with many flowers, the average natural seed setting rate is only 0.31%, and the seed germination rate is very low, so the seed resources are limited. Since the "Shen Nong's Materia Medica" in the Qin and Han Dynasties listed Dendrobium candidum as the top grade, subsequent "Compendium of Materia Medica" and "Tao Cang" have related records, and listed it as the first of the nine Chinese immortal grasses. The 2010 edition of "The Pharmacopoeia of the People's Republic of China" listed Dendrobium candidum separately. According to...

AI Technical Summary

Problems solved by technology

The research on the above documents and patents has laid a solid foundation for the large-scale planting of Dendrobium officinale to alleviate the pressure on resource supply. However, there are still deficiencies in the large-scale planting of Dendrobium candidum: seeds are used as explants, and the source of germplasm is limited. 1. Seedling cycle and reproduction coefficient failed to achieve good results at the same time; the organ regeneration method using axillary buds as explants has a short seedling cycle, but the proliferation coefficient is not high, and a large amount of explant materials are needed; existing The somatic embryo regeneration method using stem buds and terminal buds as explants shortens the seedling cultivation cycle, but it takes 160 days at the shortest; in addition, the induction efficiency of roots in the above reports is not high, only 2 to 5 per plant, not Good for transplanting

At present, there is a huge gap between supply and demand in the market, so there is an urgent need for a new method for quickly multiplying large quantities of Dendrobium officinale seedlings in a short period of time