Real-time fluorescent quantitative PCR detection system

Abstract

The invention relates to PCR detection, and concretely relates to a real-time fluorescent quantitative PCR detection system. The real-time fluorescent quantitative PCR detection system has a simple structure, is simple to operate and can simultaneously realize the fluorescent detection imaging of all samples. The real-time fluorescent quantitative PCR detection system comprises a light source, a multi-band-pass excitation optical filter, a multi-band-pass dichroscope, a lens assembly, a heat cycle system, a multi-band-pass fluorescent optical filter, a color plane array detector and an information analysis processing unit; the light source and the excitation optical filter are arranged on the horizontal axis of the dichroscope having an angle of 45DEG with a horizontal direction, and light emitted by the light source passes through the excitation optical filter reaches the dichroscope; and the lens assembly, the fluorescent optical filter and the color plane array detector are arranged on the vertical axis of the dichroscope having an angle of 45DEG with the horizontal direction from bottom to top, and the color plane array detector is connected with the information analysis processing unit.

Description

technical field [0001] The invention relates to PCR detection, in particular to a real-time fluorescent quantitative PCR detection system. Background technique [0002] The working principle of the fluorescent quantitative PCR detection system is an instrument that automatically cycles the reactant between the specified denaturation temperature, annealing temperature and extension temperature. Through the temperature cycle of denaturation, annealing and extension, the target DNA can be amplified in a short time Millions of times. At the same time, by irradiating the test tube with different wavelengths of excitation light, when the reagent in the test tube is excited to fluoresce, the optical sensor (PMT, PD, CCD, etc.) collects the fluorescence intensity signal and transmits it to the computer for real-time data display and analysis. [0003] Traditional real-time fluorescent quantitative PCR instruments (such as Chinese patent 200810120084.7) use scanning to obtain the fl...

AI Technical Summary

Problems solved by technology

[0003] Traditional real-time fluorescent quantitative PCR instruments (such as Chinese patent 200810120084.7) use scanning to obtain the fluorescence signal of the reagent. This detection method has the following disadvantages: 1) The fluorescence detection module is not fixed, and the motor drives the fiber optic probe to move. While scanning, The vibration of the optical fiber will affect the stability of the optical path, thereby affecting the detection accuracy; 2) The scanning part of the instrument has a complex mechanical structure, high cost and high quality

The common feature of the above patents is that the multi-wavelength fluorescence detection method can only be performed on the same tube of samples, and it is impossible to perform real-time fluorescence detection on all samples on the orifice plate at the same time.

To obtain the fluorescence signals of all samples, the movement of the detection device must be controlled. This detection method requires a long experiment time, low efficiency, complex mechanical structure and high cost.

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