Excitation method for multiple fluorescent dyes

Abstract

The invention discloses an excitation method for multiple fluorescent dyes, which is used for exciting multiple fluorescent dyes. The difference between the longest wavelength and the shortest wavelength in the optimal exciting light required by the multiple-fluorescent dyes respectively does not exceed 20 percent of the longest wavelength, and the multiple fluorescent dyes are excited by a broadband laser beam. According to the excitation method, the multiple fluorescent dyes are excited by one broadband laser beam, and the cost of light sources is greatly reduced, so that the excitation cost of the fluorescent dyes is reduced, and the popularization and the application of a fluorescent dye excitation technology are facilitated.

Description

technical field [0001] The present invention relates to fluorescent dyes, in particular to the excitation of multiple fluorescent dyes. Background technique [0002] DNA sequencing is an important technology in modern molecular biology research, and automatic high-speed DNA sequencing is one of the main goals pursued by this technology. In 1986, Smith proposed a four-color fluorescent marker automatic sequencing method: four fluorescent dyes with different emission wavelengths marked a series of termination fragments produced by polymerase chain reaction, separated by polyacrylamide or capillary electrophoresis, and separated by laser pair The fluorescent signals carried by the DNA fragments are excited, the fluorescent signals of different emission wavelengths are detected and recorded, processed by a computer, and finally the sequence information is obtained. Fluorescence-based detection and analysis techniques are widely used in forensic laboratories due to their advanta...

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Problems solved by technology

However, a current solid-state laser can only emit light of one wavelength. It is difficult for a single wavelength of a solid-state laser to take into account the excitation of each dye at the same time when realizing multicolor fluorescence spectrum excitation, so it is difficult for a solid-state laser to excite fluorescent dyes field promotion

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