Method for preparing fish oil by virtue of leftovers of tuna
A technology for leftovers and tuna, applied in the direction of fat oil/fat production, fat production, etc., can solve the problems of insufficient utilization of tuna leftovers, destruction of active ingredients, large energy consumption, etc. quick response
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Embodiment 1
[0025] A kind of method utilizing tuna leftovers to prepare fish oil, concrete steps are:
[0026] (1) Raw material processing: remove impurities from tuna leftovers, wash them, and then crush them to obtain blanks;
[0027] (2) Preparation of immobilized lipase: use polymethyl acrylate carrier to immobilize Candida cylindrica lipase to obtain immobilized lipase;
[0028] (3) Preliminary enzymolysis: put the billet into the enzymolysis tank, add water according to the weight ratio of billet: water = 1:2, add sodium chloride solution with a salinity of 22° to the enzymolysis tank to adjust the material in the enzymolysis tank The salinity of the liquid is 6°, and the pH of the feed liquid is controlled to be 5, then immobilized lipase is added to the enzymolysis tank, the temperature of the feed liquid is controlled at 40°C, the salinity is 4.5°, the pH is 5, and the temperature is kept for 5 hours; finally Raise the temperature to 95°C, keep it warm for 20 minutes, and filter...
Embodiment 2
[0032] A kind of method utilizing tuna leftovers to prepare fish oil, concrete steps are:
[0033] (1) Raw material processing: remove impurities from tuna leftovers, wash them, and then crush them to obtain billets;
[0034] (2) Preparation of immobilized lipase: use polymethyl acrylate carrier to immobilize Candida cylindrica lipase to obtain immobilized lipase;
[0035] (3) Preliminary enzymolysis: put the billet into the enzymolysis tank, add water according to the weight ratio of billet: water = 1:3, add sodium chloride solution with a salinity of 24° to the enzymolysis tank to adjust the material in the enzymolysis tank The salinity of the liquid is 5°, and the pH of the feed liquid is controlled to be 5.5, then immobilized lipase is added to the enzymolysis tank, the temperature of the feed liquid is controlled at 45°C, the salinity is 4°, the pH is 5.5, and the temperature is kept for 8 hours; finally Raise the temperature to 85°C, keep it warm for 22 minutes, and fil...
Embodiment 3
[0039] A kind of method utilizing tuna leftovers to prepare fish oil, concrete steps are:
[0040](1) Raw material processing: remove impurities from tuna leftovers, wash them, and then crush them to obtain blanks;
[0041] (2) Preparation of immobilized lipase: use polymethyl acrylate carrier to immobilize Candida cylindrica lipase to obtain immobilized lipase;
[0042] (3) Preliminary enzymolysis: put the billet into the enzymolysis tank, add water according to the weight ratio of billet: water = 1:4, add sodium chloride solution with a salinity of 21° to the enzymolysis tank to adjust the material in the enzymolysis tank The salinity of the liquid is 5.5°, and the pH of the feed liquid is controlled to be 6, then immobilized lipase is added to the enzymolysis tank, the temperature of the feed liquid is controlled at 35°C, the salinity is 4.3°, the pH is 6, and the temperature is kept for 3 hours; finally Raise the temperature to 90°C, keep it warm for 25 minutes, and filte...
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