PRP ribose extraction method

A nucleic acid and polysaccharide technology, applied in the field of biomedicine, can solve problems such as difficulties, low recovery rate, cumbersome extraction methods of PRP polysaccharides, etc.

Inactive Publication Date: 2013-04-24
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The traditional PRP polysaccharide extraction method is cumbersome, the recovery rate is low, and a large amount of organic reagents such as phenol and acetone are used. These organic reagents have a potential impact on the environment; Sub-consistency puts forward higher requirements. Of course, the process of extracting protein with phenol is difficult to scale up and has the disadvantages of repeated operations.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0054] 1) Concentration of Hib fermentation broth by ultrafiltration

[0055] The Hib fermentation broth was centrifuged at 8000rpm for 20min to remove the bottom of the bacteria, and then passed through a 0.22um hollow fiber. The filtrate was concentrated by 20-fold ultrafiltration with a 50KDa membrane bag. The concentrated solution was used for the separation and purification of the Q column, and 4L of the fermentation broth was fermented experimentally. After concentration by ultrafiltration, 200 ml of concentrated solution was obtained.

[0056] 2) Q-sepharose column separation and purification ultrafiltration concentrate

[0057] The size of the Q-sepharose column is 16*100cm, provided by GE, and a larger volume separation column can also be selected, depending on the experiment; for the 16*100cm separation column, the sample volume for each experiment is 100ml, and the ultrafiltration concentration The solution was carried out twice; before loading the sample, equilibr...

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Abstract

The invention relates to a Haemophilus influenzae type B polyribosyl ribitol phosphate purification method, which comprises the following steps: 1) removing thallus in a Haemophilus influenzae type fermentation broth to obtain a fermented supernatant; and 2) removing nucleic acid and protein in the fermented supernatant to obtain PRP polysaccharide.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for preparing PRP ribose. Background technique [0002] Haemophilus influenzae type b (Hib) is the main pathogenic factor causing meningitis and otitis media in infants and young children. repeat unit. Studies have found that anti-PRP polysaccharide antibodies can effectively reduce Hib infection, so PRP-based polysaccharide vaccines or conjugate vaccines have become the main method to prevent Hib infection. [0003] Hib conjugate vaccine is the earliest and most successful conjugate vaccine developed in the world. The successful development of this type of conjugate vaccine has solved the shortcoming that polysaccharide vaccines cannot effectively stimulate the immune response of infants. With the development of conjugate vaccine technology, there are now many Pharmaceutical companies have developed different types of Hib conjugate vaccines; [0004] Haemophilus ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 李军强
Owner TIANJIN TASLY PHARMA CO LTD
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