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Method for preparing microbial flocculant form potato starch wastewater as raw material

A technology for microbial flocculant and potato starch, which is applied in the field of preparing microbial flocculants, can solve the problems of secondary environmental pollution, large temperature influence, long time consumption, etc., and achieves the effects of high flocculation activity, good environmental benefits and low price.

Inactive Publication Date: 2014-05-21
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If it is discharged directly, it will cause environmental hazards such as eutrophication of water bodies.
[0003] At present, the treatment methods of potato starch wastewater include physical chemical methods and biological treatment methods, but various problems will be encountered in practical application, such as the use of traditional flocculants such as polyacrylamide, ferric chloride, aluminum sulfate, not only causing secondary pollution to the environment secondary pollution, and poses a threat to human health; biological treatment methods include anaerobic biological treatment and aerobic biological treatment, but biological treatment is greatly affected by temperature, and potato starch processing in my country is generally from September to March of the next year. The temperature in northern my country is low, so the biological treatment efficiency is low, time-consuming and takes up a lot of space

Method used

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  • Method for preparing microbial flocculant form potato starch wastewater as raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 A kind of method using potato starch waste water as raw material to prepare microbial flocculant comprises the following steps:

[0022] ⑴ Screen out the microbial flocculant strain, which is Candida anglica , and its preservation number in the General Microbiology Center of China Microbiological Culture Collection Management Committee is CGMCC No: 7032 (preservation unit address: Institute of Microbiology, Chinese Academy of Sciences, No. 1, No. 1 Beichen West Road, Chaoyang District, Beijing; preservation date: December 2012 24th).

[0023] Wherein: bacterial strain refers to the activated sludge of the secondary settling tank of sewage treatment plant as strain isolation material, and potato dextrose agar medium (PDA) is used as separation medium, adopts conventional plate separation method to obtain multiple pure bacterial strains, the multiple pure bacterial strains The strains were inoculated in potato glucose liquid culture medium with 1-2 ring in...

Embodiment 2

[0029] Example 2 A kind of method using potato starch waste water as raw material to prepare microbial flocculant comprises the following steps:

[0030] ⑴ Screen out the microbial flocculant strain, which is Candida anglica , and its preservation number in the General Microbiology Center of China Microbiological Culture Collection Management Committee is CGMCC No: 7032 (preservation unit address: Institute of Microbiology, Chinese Academy of Sciences, No. 1, No. 1 Beichen West Road, Chaoyang District, Beijing; preservation date: December 2012 24th).

[0031] Among them: the identification of strains and strains is the same as Example 1 .

[0032] ⑵ Candida anglica The preparation of the primary seed solution of the strain: the Candida anglica The slant strains were inoculated in 1-2 rings of potato dextrose liquid medium, and cultured for 24 hours at 30°C with a shaker speed of 180 r / min to obtain a cell concentration of 1.2×10 6 individual / mL primary seed solution...

Embodiment 3

[0036] Example 3 A kind of method using potato starch waste water as raw material to prepare microbial flocculant comprises the following steps:

[0037] ⑴ Screen out the microbial flocculant strain, which is Candida anglica , and its preservation number in the General Microbiology Center of China Microbiological Culture Collection Management Committee is CGMCC No: 7032 (preservation unit address: Institute of Microbiology, Chinese Academy of Sciences, No. 1, No. 1 Beichen West Road, Chaoyang District, Beijing; preservation date: December 2012 24th).

[0038] Among them: the identification of strains and strains is the same as Example 1 .

[0039] ⑵ Candida anglica The preparation of the primary seed solution of the strain: the Candida anglica The slant bacteria were inoculated in ring 1-2 of potato dextrose liquid medium, and cultured for 36 hours at 28°C with a shaker speed of 150 r / min to obtain a cell concentration of 1.2×10 6 individual / mL primary seed solution...

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Abstract

The invention relates to a method for preparing a microbial flocculant form potato starch wastewater as a raw material. The method comprises the following steps of: (1) screening a microbial flocculant strain Candida anglica; (2) preparing primary seed solution of the strain Candida anglica; (3) preparing a fermentation medium; (4) preparing fermentation liquor with flocculation activity; and (5) purifying, extracting, freezing and drying the fermentation liquor with flocculation activity to obtain a crude extract of the flocculant. The method is simple in process, short in fermentation period and easy to implement, has remarkable environmental and economic benefits, and is suitable for industrial production and popularization.

Description

technical field [0001] The invention relates to a method for preparing a microbial flocculant, in particular to a method for preparing a microbial flocculant by using potato starch wastewater as a raw material. Background technique [0002] my country is a large potato producing country, and its planting area is the second in the world. It is the main processing raw material of industrial starch together with wheat and corn. In recent years, with the rapid development of the starch processing industry, my country processes about 3 million tons of potatoes annually and produces about 8 million tons of wastewater. Potato starch wastewater is a high-concentration organic wastewater, containing various sugars, various amino acids, fats, organic acids, vitamins and enzymes, etc., with high chemical oxygen demand (COD), about 8000~40000 mg / L, biochemical It has good properties and has the potential to be used as a fermentation medium. If it is discharged directly, it will cause ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02C12R1/72
Inventor 贠建民颜东方张紊玮艾对元
Owner GANSU AGRI UNIV
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