Method for continuously producing L-ornithine composite salt by enzyme immobilization
A technology of enzyme immobilization and ornithine, which is applied in the direction of immobilization on/in organic carriers, fermentation, etc., can solve the problems of restricting the promotion and popularization of production methods, complex strain control and production control, and acid production rate of strains No high problems, achieve the effect of reduced energy consumption, stable quality, and simplified production process
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Embodiment 1
[0023] ① Soak the macroporous adsorption resin and 10% sodium carbonate solution for 12 hours, filter, wash the filtered resin with pure water, and then mix according to the mass ratio of resin: sodium carbonate: pure water = 1:0.3:30 Form a solution, adjust the pH value of the solution to 9, stir and lower the temperature to ≤-5°C, slowly add sodium carbonate with a mass of 0.3% of the resin dropwise, and after holding for 2 hours, add sodium carbonate with a concentration of 1% and a mass of 0.001% of the mass of the resin Enzyme aqueous solution, warmed up to 0°C for 10 hours, filtered out the resin, washed three times with 10 times pure water to obtain immobilized enzyme resin;
[0024] ②Put 1000L of deionized water into a 2000L reactor, add 300kg of L-arginine while stirring, and raise the temperature to 25°C to obtain an aqueous solution of L-arginine with a mass concentration of 300g / l;
[0025] ③Put 150g of manganese acetate into the reaction kettle, stir for 10 minute...
Embodiment 2
[0028] ① Soak the macroporous adsorption resin and 10% sodium carbonate solution for 12 hours, filter, wash the filtered resin with pure water, and then mix according to the mass ratio of resin: sodium carbonate: pure water = 1:0.5:60 Form a solution, adjust the pH value of the solution to 11, stir and lower the temperature to ≤-5°C, slowly add cyanogen bromide with a mass of 0.5% of the resin, and keep it warm for 2 hours, add a concentration of 1%, and a mass of 0.003% of the mass of the resin The enzyme aqueous solution was heated to 5°C for 13 hours, the resin was filtered out, and washed three times with 10 times pure water to obtain the immobilized enzyme resin;
[0029] ②Put 1000L of deionized water into a 2000L reactor, add 400kg of L-arginine while stirring, and raise the temperature to 40°C to obtain an aqueous solution of L-arginine with a mass concentration of 400g / l;
[0030] ③Add 200g of manganese acetate into the reaction kettle, stir for 10 minutes, add 8 kg of...
Embodiment 3
[0033] ① Soak the macroporous adsorption resin and 10% sodium carbonate solution for 12 hours, filter, wash the filtered resin with pure water, and then mix according to the mass ratio of resin: sodium carbonate: pure water = 1:0.8:100 To form a solution, adjust the pH value of the solution to 13, stir and lower the temperature to ≤-5°C, slowly add cyanogen bromide with a mass of 0.8% of the resin, and keep it for 2 hours, add a concentration of 1%, and a mass of 0.005% of the mass of the resin The enzyme aqueous solution was heated to 10°C for 16 hours, the resin was filtered out, and washed three times with 10 times pure water to obtain the immobilized enzyme resin;
[0034] ②Put 1000L of deionized water into a 2000L reaction kettle, add 500 kg of L-arginine while stirring, and raise the temperature to 55°C to obtain an aqueous solution of L-arginine with a mass concentration of 500g / l;
[0035] ③Put 250g of manganese acetate into the reaction kettle, stir for 10 minutes, ad...
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