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Oil production microbe culture method

A technology for oleaginous microorganisms and microbial cultivation, which is applied in the field of oleaginous microorganism cultivation, can solve problems such as lack of universal practicability, and achieve the effects of shortening the fermentation cycle, improving sugar utilization efficiency, and reducing costs

Inactive Publication Date: 2013-04-03
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technique allows us to produce valuable chemical compounds from biomass by culturing them under specific conditions called nutrients (such as sugars). By breaking down these substances into smaller molecules we created novel ways to make better types of useful organics like biofuels. Additionally, this approach helps improve both yield and quality of crops while reducing their environmental impact through waste disposal methods such as burning at harvest sites. Overall, this innovative solution provides various potential applications including making fuel alternatives and producing specialty products.

Problems solved by technology

This patented method addresses issues related to enhanced metabolite formation caused by excessive consumption of sugars like sugar alcohol and starch due to glycosyltransferases involved in lipid biomass conversion processes. Existing techniques involve either adjustments based upon specific substrate properties or require expensive equipment.

Method used

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  • Oil production microbe culture method

Examples

Experimental program
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Effect test

Embodiment 1

[0035] 1) Prepare cellobiose and xylose into a mixed sugar solution, cellobiose 47g / L, xylose 23g / L, add 0.5g / L yeast powder, 1.0g / L ammonium sulfate, 1.0g / L heptahydrate sulfuric acid Magnesium, 1.0g / L dipotassium hydrogen phosphate, the balance is water, pH 5.8, and the obtained culture medium is sterilized at 121°C for 15 minutes before use;

[0036] 2) Lipomyces starkeyi AS 2.1560 was cultured in YEPD liquid medium (glucose 20g / L, yeast powder 10g / L, peptone 10g / L) at 30°C and 200rpm for 40h to obtain seed liquid;

[0037] 3) Add the oleaginous microorganism seed solution prepared in step (2) to the culture medium described in step (1) (containing 10 7 living cells), the inoculum size was 10% (v / v), and cultured under aeration at 30°C for 108h;

[0038] 4) Terminate the fermentation. At this time, the residual cellobiose and xylose concentrations in the fermentation broth are respectively 0.5g / L and 0g / L. During the fermentation process, the cellobiose and xylose concentr...

Embodiment 2

[0041] 1) Prepare cellobiose and xylose into a mixed sugar solution, cellobiose 35g / L, xylose 35g / L, add 0.5g / L yeast powder, 0.5g / L ammonium sulfate, 1.0g / L heptahydrate sulfuric acid Magnesium, 7.0g / L potassium dihydrogen phosphate, 2.0g / L disodium hydrogen phosphate, the balance is water, pH 5.5, and the obtained culture medium is sterilized at 121°C for 15 minutes before use;

[0042] 2) Sporobolomyces roseus IAM 13481 (purchased from Japan JCM Strain Collection Center) in liquid seed medium (glucose 40g / L, ammonium sulfate 5g / L, yeast powder 0.5g / L, potassium dihydrogen phosphate 1g / L, Magnesium sulfate heptahydrate 0.5g / L), 30 ℃, 200rpm shaking culture 24h, obtain seed solution (contain 5 * 10 6 living cells);

[0043] 3) Add the oleaginous microorganism seed solution prepared in step (2) to the medium described in step (1), the inoculum size is 15% (v / v), and aerated culture at 30° C. for 103 h;

[0044] 4) Terminate the fermentation. At this time, the residual cellob...

Embodiment 3

[0046] 1) Prepare cellobiose and xylose into a mixed sugar solution, cellobiose 23g / L, xylose 47g / L, add 1.0g / L yeast powder, 2.0g / L ammonium sulfate, 0.5g / L heptahydrate sulfuric acid Magnesium, 2.0g / L dipotassium hydrogen phosphate, the balance is water, pH 4.0, and the obtained culture medium is sterilized at 121°C for 15 minutes before use;

[0047] 2) Geotrichum robustum CICC 1256 (purchased from China Industrial Microorganism Culture Collection Management Center) in YEPD liquid medium (glucose 20g / L, yeast powder 10g / L, peptone 20g / L), 28°C, 200rpm Shake culture for 28 hours to obtain seed liquid (containing 8×10 5 living cells);

[0048] 3) Add the oleaginous microorganism seed solution prepared in step (2) to the culture medium described in step (1), inoculum size 5% (v / v), and aerobically culture at 28° C. for 120 h;

[0049] 4) Terminate the fermentation. At this time, the residual cellobiose and xylose concentrations in the fermentation broth are respectively 0.5g...

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Abstract

The invention discloses a new oil production microbe culture method, wherein a mixture of cellobiose and xylose is adopted as a main carbon source substance, other necessary nutrients are added, gas introduction is performed to culture oil production microbe, and microbe oil is prepared, wherein the mixture of the cellobiose and the xylose can be prepared through lignocelluloses raw material hydrolysis, the lignocelluloses raw material is subjected to complete hydrolysis to usually obtain a hydrolyzate adopting glucose and xylose as main ingredients, a glucose effect exists when the hydrolyzate is used for microbe culture, and a fermentation period is long. According to the present invention, the glucose effect is avoided, a culture period is shortened, production efficiency and raw material utilization rate are increased, cost is reduced, technical economy of the microbe oil is improved, and the method can be applicable for microbe oil and biodiesel preparation through biomass resource conversion.

Description

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Claims

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Application Information

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Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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