Refining and extracting method of dextran by membrane separation

A purification method and plasma-substituting technology, applied in the field of membrane separation, can solve the problems of narrow and high-purity products, inability to obtain molecular weight distribution, difficulty in filtration, etc., achieve high economic and social benefits, safe and reliable product efficacy, and improve product quality. Effect

Active Publication Date: 2013-03-27
JIANGSU JIUWU HITECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented method uses two different techniques: one separates out certain substances from blood by filtration or centrifuging them into smaller particles called platelets; another involves adding these tiny parts like drugs for better results. These methods are effective at producing pure products while also improving their properties such as reducing side-effects caused by other ingredients used during production.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding ways to safely utilizing plasmas instead of drugs due to their potential harmfulness towards healthy tissues during treatments like bleeding disorders (hemophilia). Current methods involve extracting plasma proteins directly through extraction processes which may lead to decomposition and denaturations of these valuable components.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take 30L of plasma substitute (hydroxyethyl starch) crude product solution with an average molecular weight of 120kDa, a purity of 70.3%, and a molecular weight range of 500-200,000Da, and use an alumina microfiltration membrane with an average pore size of 100nm for pretreatment and filtration. The operating pressure is 0.8 MPa , the membrane surface flow rate is 5 m / s, and the large particles and bacteria in the plasma substitute (hydroxyethyl starch) solution are removed.

[0028] Next, the obtained permeate is sent to a zirconia ultrafiltration membrane with an average pore size of 20 nm for primary filtration to remove high molecular weight components. The operating pressure is 0.5 MPa and the membrane surface velocity is 10 m / s.

[0029] The obtained permeate enters the titanium oxide ultrafiltration membrane with an average pore size of 2nm, the operating pressure is 0.5MPa, the membrane surface flow rate is 5 m / s, and the temperature is 40°C to remove small molec...

Embodiment 2

[0033] Take 30L of plasma substitute (hydroxyethyl starch) crude product solution with an average molecular weight of 200kDa, a purity of 72.4%, and a molecular weight range of 10-300kDa, and use an aluminum oxide microfiltration membrane with an average pore size of 500 nm for pretreatment and filtration. The operating pressure is 0.5 MPa, membrane surface flow velocity 10 m / s, to remove large particles and bacteria in the plasma substitute (hydroxyethyl starch) solution.

[0034] Next, the obtained permeate was sent to a zirconia ultrafiltration membrane with an average pore size of 50 nm for primary filtration to remove high molecular weight components. The operating pressure was 0.8 MPa and the membrane surface velocity was 5 m / s.

[0035] The obtained permeate enters the titanium oxide ultrafiltration membrane with an average pore size of 10nm, the operating pressure is 0.8 MPa, the membrane surface flow rate is 0.07 m / s, and the temperature is 20°C to remove small molecul...

Embodiment 3

[0039] Take 30 L of the crude plasma substitute solution (hydroxyethyl starch) with an average molecular weight of 150 kDa and a molecular weight range of 50-300 kDa, and use a porous titanium microfiltration membrane with an average pore diameter of 800 nm for pretreatment and filtration. The operating pressure is 0.1 MPa. The flow rate is 0.07 m / s to remove large particles and bacteria in the plasma substitute (hydroxyethyl starch) solution.

[0040] Next, the obtained permeate was sent to a zirconia ultrafiltration membrane with an average pore size of 100 nm for primary filtration to remove high molecular weight components. The operating pressure was 0.1 MPa and the membrane surface velocity was 0.07 m / s.

[0041] The obtained permeate enters the zirconia ultrafiltration membrane with an average pore size of 20nm, the operating pressure is 0.8 MPa, the membrane surface flow rate is 0.07 m / s, and the temperature is 80 ℃ to remove small molecular components in the feed liquid...

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Abstract

The invention belongs to the technical field of membrane separation, and relates to a refining and extracting filtering preparation process of dextran. The method comprises the steps of: first, filtering a crude product liquor of dextran by a micro-filtration membrane; second, filtering the filtrate in the first step by a first ultrafiltration membrane; and third, filtering the filtrate in the second step by a second ultrafiltration membrane, wherein trapped fluid is the refined dextran, the mean pore size of the first ultrafiltration membrane is 20-100nm, and the mean pore size of the second ultrafiltration membrane is 2-20mn or the molecular weight cut-off is 3-100kDa. According to the invention, the membrane separation technology and the pharmaceutical process are combined to filter and refine a dextran raw material, so that a dextran product with narrower distribution of molecular weight is high in purity, is safe and reliable in curative effect and has high economic and beneficial benefits, and the product quality is improved.

Description

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Claims

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Application Information

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Owner JIANGSU JIUWU HITECH
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