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Method for obtaining explants for tissue culture of bamboo

An acquisition method and explant technology, applied in the field of acquisition of bamboo cultivation explants, can solve the problems of low pollution rate, high induction rate, etc., and achieve the effects of low pollution rate, strong germination ability of cluster buds, and improved utilization rate of branches

Active Publication Date: 2014-01-08
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for obtaining explants of bamboo tissue culture with low pollution rate, high induction rate, low cost and simple operation for the deficiency of obtaining explants in the existing bamboo tissue culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Acquisition of tissue cultured explants of bamboo

[0024] In mid-February, select and measure the excellent green bamboo bushes and plant them in a container with a substrate. The container specification is 50 cm in diameter and 60 cm in height. The substrate in the container is 10 parts of topsoil, 3 parts of wood chaff, and decomposed organic 1 share of fertilizer, and the row spacing of bamboo clumps is 0.7m×0.7m. After 6 months of planting, cut off the mother bamboo at a distance of 1.3m~1.5m from the ground, bury and apply 100~120g of compound fertilizer to each clump of mother bamboo, and cut off the old branches on the mother bamboo in June and February of the next year. When it is too vigorous, loosen the soil around the container and remove the roots once. Cut semi-lignified branches from the mother bamboo several times every year from May to November, select branches with a diameter of 0.1-0.3 cm and 3-6 nodes, and cut off the leaves. Cut every ...

Embodiment 2

[0025] Example 2: Acquisition of tissue culture explants of Diaosi bamboo

[0026] In the first ten days of March, select and measure the excellent hanging silk bamboo clusters and plant them in the container with the substrate. The container specification is 80cm in diameter and 50cm in height. 2 parts of fertilizer, and the row spacing of bamboo clumps is 1.0m×1.3m. In the first ten days of September of that year, cut off the mother bamboo at a distance of 1.5m~1.8m from the ground, and apply 100~200g of compound fertilizer to each clump of mother bamboo in June, and cut off the old branches on the mother bamboo in July and March of the following year. When the bamboo shoots grow too vigorously, loosen the soil around the container and remove the roots once in March. Cut semi-lignified branches from the mother bamboo several times a year from May to November, select branches with a diameter of 0.2-0.4 cm and 4-6 nodes, and cut off the leaves. Cut every 3 nodes into a ste...

Embodiment 3

[0027] Example 3: Acquisition of Tissue Culture Explants of Phyllostachys edulis

[0028] In the middle of March, select the excellent powder unit bamboo clumps that have been measured and plant them in a container with a substrate. The container specification is 70 cm in diameter and 55 cm in height. 1 part of organic fertilizer, and the row spacing of bamboo clumps is 0.8m×1.0m. After 7 months of planting, cut off the mother bamboo at a distance of 1.3m~1.5m from the ground, bury and apply 100~200g of compound fertilizer to each cluster of mother bamboo in March, and cut off the old branches on the mother bamboo in August and next March. When the new bamboo shoots grow too vigorously, loosen the soil around the container and remove the roots once in February. Cut semi-lignified branches from the mother bamboo several times a year from May to November, select branches with a diameter of 0.-0.3 cm and 3-6 nodes, and cut off the leaves. Cut every 3 nodes into a stem section...

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Abstract

The invention discloses a method for obtaining explants for tissue culture of bamboo. The method comprises the steps: selecting tested fine branch stumps of bamboo groves, and planting the branch stumps in a container; cutting off the mother bamboos growing up in current year at a distance of 1.3-1.8m to the ground in August to October; applying fertilizer and trimming regularly and cutting the roots according to conditions so as to control the growth of the bamboos; clipping half-lignified branches from the mother bamboos for a plurality of times in May to November annually, cutting off the leaves and trimming the branches into stems; washing and disinfecting the stems through liquid detergent, tap water, alcohol, corrosive sublimate and sterile water; and finally trimming the stems again to obtain sterile explants for tissue culture of bamboo. Through the method, explants in large quantity with good quality and low contamination rate can be obtained, and the cluster buds have strong germination ability. The method is simple and convenient in operation and low in production cost. The method is suitable for industrialized seedling production of bambusa texlitis, bambusa chungii and dendrocalamus minor, and has good economic, social and ecological effects.

Description

Technical field [0001] The present invention is the field of non -propagation technology of plants, involving a method of acquisition of a bamboo cultivation outside implant. Background technique [0002] Bamboo plants are an important part of my country's forest resources. It has the characteristics of fast growth, early production, high yield, and wide use of use. At the same time, it has good water conservation and soil and water conservation functions. It is a plant with good economic and ecological benefits.Bamboo plants are rarely blooming and not easy to be strong, so they do not use seed seedlings, but use asexual breeding method to breed small seedlings.Seed seedlings mainly include cuttings and organizational training.However, cuttings reproduction due to the low utilization rate of mother bamboo, which has a large damage to the original bamboo forest resources and low reproduction rates. It is difficult to produce seedlings in large quantities.Small, high reproductive ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00
Inventor 郝海坤黄大勇黄开勇陈琴黄志玲李立杰徐振国
Owner GUANGXI FORESTRY RES INST
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