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High-activity strain capable of realizing malolactic conversion

A technology of malolactic acid and high activity, applied in the field of microorganisms, can solve the problems of low fermentation temperature, reducing the acid-reducing effect of lactic acid bacteria, low pH value, etc., and achieve high stress resistance, strong malolactic acid conversion activity, and soft taste Effect

Active Publication Date: 2013-11-06
福建省春秋农林科技有限公司
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  • Abstract
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Problems solved by technology

However, the high content of sulfur dioxide and alcohol concentration in fruit wine, low pH value, and low fermentation temperature all inhibit the growth of lactic acid bacteria, thereby greatly reducing the acid-reducing effect of lactic acid bacteria

Method used

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  • High-activity strain capable of realizing malolactic conversion
  • High-activity strain capable of realizing malolactic conversion
  • High-activity strain capable of realizing malolactic conversion

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Embodiment Construction

[0019] Lactobacillus plantarum subsp. of the present invention ( Lactobacillus plantarum subsp. plantarum ) RF17 is against Lactobacillus plantarum R23 ( Lactobacillus plantarum ) was obtained by ultraviolet nitrosoguanidine compound mutagenesis.

[0020] 1. The acquisition of the strain RF17

[0021] The concentration of 2mL is 2.0~3.0×10 7 The bacterial suspension of cfu / mL Lactobacillus plantarum R23 is centrifuged to collect the bacterial cells, and nitrosoguanidine is added to the collected bacterial cells to form a nitrosoguanidine solution, and the nitrosoguanidine in the nitrosoguanidine solution The concentration was 2 mg / mL, and then placed on a shaker at 28 °C at 150 r / min for 60 min. After the shaking was completed, 25% sodium thiosulfate solution was added according to 2.5 times the volume of the nitrosoguanidine solution to terminate the reaction, and then centrifuged at 12000 rpm for 10 min. Discard the supernatant, wash the bacteria three times with 5mL PBS...

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Abstract

The invention provides a high-activity strain capable of realizing malolactic conversion. The high-activity strain is Lactobacillus plantarum subsp. Plantarum RF17 which is preserved in China Center for Type Culture Collection in Wuhan University on Mar.16, 2012, with the preservation number of CCTCC NO: M 2012085. The high-activity strain capable of realizing malolactic conversion, provided by the invention has the advantages that by utilizing the strain, namely the Lactobacillus plantarum subsp. Plantarum RF17, the content of malic acid in fruit wine and fruit juice can be reduced more efficiently, content of lactic acid, volatile ester and the like in the fruit wine and the fruit juice can be increased, so that the fruit wine and the fruit juice is more gentle and coordinated in mouthfeel, and is sweet-scented in flavor, a solid foundation is laid to improve quality of the fruit wine and the fruit juice, and therefore a novel strain source is provided for the research on the biological acid-degradation and lactic acid fermentation of the fruit wine.

Description

【Technical field】 [0001] The present invention relates to a kind of microorganism, relate in particular to a kind of malolactic acid conversion high vigor strain, and this bacterial strain is Lactobacillus plantarum subspecies ( Lactobacillus plantarum subsp. plantarum ) RF17. 【Background technique】 [0002] High content of malic acid in fruit wine will lead to sour body and strong roughness. Malo-lactic acid fermentation is that lactic acid bacteria use dicarboxylic L-malic acid as a substrate, and convert it into monocarboxylic L-lactic acid under the catalysis of malic acid-lactic acid enzyme. and CO 2 This process can soften the wine body and play a role in flavor modification. It is one of the important biological acid reduction processes for brewing high-quality fruit wine. However, the high content of sulfur dioxide and alcohol concentration in fruit wine, low pH value, and low fermentation temperature all inhibit the growth of lactic acid bacteria, thereby greatly ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/25
Inventor 何志刚任香芸梁璋成李维新林晓姿魏巍
Owner 福建省春秋农林科技有限公司
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