Method for constructing asthma model of nonhuman mammal

Abstract

The invention provides a method for constructing an asthma model of a nonhuman mammal. The method comprises the following steps of: 1, injecting an effective quantity of phosphate buffer solution (PBS) containing egg white and aluminum hydroxide gel into the abdominal cavity of the nonhuman mammal in the 1st day and the 7th day, and slowly dripping an effective quantity of PBS containing egg white and lipopolysaccharide from the guttural wall of the nonhuman mammal at the same time; and 2, atomizing the PBS containing the egg white continuously from the 13th day to the 15th day, and performing respiratory tract sensitization of antigens on the nonhuman mammal. By the method, the asthma model of the nonhuman mammal of which the airway has increased neutrophil granulocytes is successfully constructed, the probability of neutrophil granulocyte increase caused by airway injury is eliminated, and the modeling time is shortened.

Description

technical field [0001] The invention relates to the field of constructing asthma animal models, in particular to a method for constructing non-human mammalian asthma models. Background technique [0002] Bronchial asthma is a chronic non-specific inflammation of the airways, with a large number of patients worldwide, and it takes a lot of manpower and material resources to control asthma attacks. In order to alleviate the suffering of patients as much as possible, it is necessary to construct disease animal models and study the pathogenesis of asthma. In the past, it was believed that airway eosinophil infiltration caused by allergen-induced Th1 / Th2 response imbalance is an important pathological feature of asthma, and such patients are sensitive to hormone therapy. Most of the models constructed by the prior art lead to the increase of eosinophils. The commonly used method of constructing this type of model is: intraperitoneal injection of chicken egg protein on the 1st d...

AI Technical Summary

Problems solved by technology

Although the existing technology can successfully construct related asthma models, there are three sensitizations, respectively on the 0th, 7th, and 14th day, and respectively on the 21st day, for two consecutive weeks, the modeling time is relatively long, and intranasal drip of lipopolysaccharide If the amount is too large, the possibility of neutrophil increase caused by airway damage cannot be ruled out. It does not meet the pathogenesis of asthma because allergens induce an imbalance in the immune response in the body

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