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Maize flooding stress response zmERF5 gene promoter

A flooding stress response and promoter technology, applied in the field of the zmERF5 gene promoter field of corn flooding stress response, to achieve the effect of improving waterlogging tolerance

Inactive Publication Date: 2013-01-16
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, the cloning of the maize ERF promoter has not yet been reported

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0049] The zmERF5 promoter was cloned into the transformation vector, followed by a Tnos terminator to construct a transformation vector. The transformed target gene was cloned between the zmERF5 promoter and the Tnos terminator in the correct direction to construct a transformation vector. Then, the target gene is transformed into recipient cells by gene gun bombardment method or Agrobacterium-mediated method, and transgenic plants are obtained by technical means such as tissue culture. Under waterlogging and flooding conditions, the zmERF5 promoter can promote the massive expression of the target gene in the root of the plant.

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Abstract

The invention relates to a maize flooding stress response zmERF5 gene promoter, belongs to the technical field of plant gene engineering and is characterized by disclosing a DNA sequence of the zmERF5 gene promoter. The promoter is characterized by comprising cis-elements, namely two AuxRR-Cores, a CE3, four CGTCA-motifs and two MBS sites, wherein the cis-elements are related to plant hormones and anaerobic stress responses. The promoter is a tissue-specific promoter only expressed in the root system and an inductive promoter efficiently expressed under flooding induction. The promoter is associated with target genes with insect resistance, disease resistance, adversity resistance and the like, so that the insect-resistant, disease-resistant and adversity-resistant capabilities of the roots of transgenic plants can be improved under flooding and waterlogging conditions. Meanwhile, the promoter is only expressed in the roots so that food safety problems cannot occur when the overground parts of the transgenic plants are eaten. The zmERF5 promoter has an excellent application prospect in the waterlogging-resistant genetic improvement of xerophilous plants.

Description

Technical field: [0001] The invention relates to a zmERF5 gene promoter responding to waterlogging stress in maize, belonging to the technical field of plant genetic engineering. Background technique: [0002] The average area of ​​farmland affected by floods in my country is 9.56 million hectares every year, and in severe years, the affected area is more than 15 million hectares. In Asia, flood disasters have caused great losses to corn production. In South Asia alone, more than 15% of the corn planting area suffers damage to varying degrees every year. In India, floods have reduced maize production by 25-30% per year. Due to abundant rainfall and imperfect drainage and irrigation systems in southern my country, spring maize in low-lying areas often suffers from waterlogging, resulting in a severe reduction in maize production. Waterlogging damage has become one of the main restrictive factors for high and stable maize yield in southern my country. Therefore, the cloning...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/10C12N15/82A01H5/00
Inventor 杜何为黄敏张祖新陈威黄育刚范金香吴闯
Owner YANGTZE UNIVERSITY
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