Compound biological agent for deodorizing public toilets, and preparation method and application of compound biological agent
A technology of biological preparations and public toilets, applied in the field of applied biology, can solve the problems that it is difficult to give full play to the complementary deodorization performance of plant extracts and microbial agents, the price of raw materials is expensive, and the deodorization effect is slow, so as to achieve in-situ source control of odor , reduce the intensity of odor release, and improve the effect of toilet environment
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Embodiment 1
[0044] (1) Quantitatively prepare the MRS solid medium that has been sterilized at high temperature (121°C, 30 minutes). The formula of the MRS solid medium is 10g of peptone, 10g of beef extract, 5g of yeast extract, 5g of glucose, 5g of sodium acetate, and citric acid. Diammonium 2g, Tween 801mL, magnesium sulfate 0.58g, manganese sulfate 0.05g, dipotassium hydrogen phosphate 2g, agar 15-20g, add water to 1000mL, pH 6.2.
[0045] (2) The strains ZW and SR were respectively inserted into the sterilized (121°C, 30 minutes) MRS solid slant medium to be activated for later use.
[0046] (3), the liquid medium that has been subjected to high-temperature sterilization (121°C, 30 minutes) is quantitatively set aside, and the formula of the liquid medium is peptone 10g, beef extract 10g, yeast extract 5g, glucose 5g, sodium acetate 5g, dicitric acid Ammonium 2g, Tween 801mL, magnesium sulfate 0.58g, manganese sulfate 0.05g, dipotassium hydrogen phosphate 2g, add water to 1000mL, pH ...
Embodiment 2
[0054] Add 10ml of liquid compound biological agent to a beaker (1L) with 400g of fresh feces, and seal the beaker with plastic wrap; at the same time, do a control without adding bacterial agents, and each group of experiments is set to repeat three times, and the odor intensity and ammonia concentration are measured after 5 days , hydrogen sulfide concentration and TVOC concentration, the measurement results are as follows in Table 1:
[0055] Table 1: Deodorizing effect of compound biological preparations
[0056]
[0057] It can be seen from the above table that after adding bacterial agents to the feces, the odor intensity, ammonia concentration, hydrogen sulfide concentration and TVOC concentration were all significantly lower than those of the control, of which the odor intensity decreased by 38.8%, the ammonia concentration decreased by 44.3%, and the hydrogen sulfide concentration decreased by 38.8%. 33.8% reduction, TVOC concentration reduction 35.8%.
Embodiment 3
[0059] Add 100g of solid compound biological agent (after activation) to a beaker (2L) with 1000g of fresh feces, mix it with the feces, and seal the beaker with plastic wrap; at the same time, do a control without adding bacterial agents, and set three replicates for each group of experiments , measure stench intensity, ammonia concentration, hydrogen sulfide concentration and TVOC concentration after 5 days, measurement result is as follows table 2:
[0060] Table 2: Deodorizing effect of bacteria agent
[0061]
[0062] It can be seen from the above table that after adding bacterial agents to the feces, the odor intensity, ammonia concentration, hydrogen sulfide concentration and TVOC concentration were all significantly lower than those of the control, of which the odor intensity decreased by 41%, the ammonia concentration decreased by 49.6%, and the hydrogen sulfide concentration decreased by 41%. decreased by 40.1%, and TVOC concentration decreased by 38.7%.
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