Vitrification ultralow-temperature preserving method for agapanthus embryogenic callus

Abstract

The invention relates to the field of ultralow-temperature preservation, and in particular relates to a vitrification ultralow-temperature preserving method for agapanthus embryogenic callus, after low temperature-hypertonic pretreatment, the agapanthus embryogenic callus is sequentially dehydrated in loading solution and vitrification solution; and finally, the dehydrated agapanthus embryogenic callus is added with the vitrification solution again, and then quickly put in liquid nitrogen to be preserved. The agapanthus embryogenic callus is preserved at ultralow temperature through specially designing the treatment methods, conditions and reagents of a vitrification ultralow temperature preserving process, so a technical guarantee is provided for preserving agapanthus genetic resources, a new effective method is provided for somatic embryogenetic seedlings and new product cultivating materials, and a guiding function is achieved for preserving plant resources which grow in tropical zones or contain high water contents and for completing an asexual propagation technical system.

Description

technical field [0001] The invention relates to the field of cryopreservation, in particular to a method for vitrifying cryopreservation of embryogenic callus of Agapanthus lilifolia. Background technique [0002] Agapanthus praecox ssp.orientalis, also known as 'blue lily', is a perennial rhizome flower native to southern Africa. Agapanthus has beautiful leaf shape, beautiful flower posture, elegant flower color, long flowering period, strong resistance to stress, and strong ornamental characteristics. From the use of medicinal components of its leaves and rhizomes to ornamental flowers, from garden cultivation to cut flowers Production, with high ornamental value and application value, is high-grade fresh cut flowers, potted flowers and coastal park ground covering materials in western developed countries, and is also the most expressive love flower besides roses. In order to rapidly propagate the introduced excellent germplasm resources, it is necessary to carry out the ...

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Problems solved by technology

At home and abroad, research on cryopreservation technology has been carried out on different explants of more than 200 kinds of plants. However, some plants (such as tropical plants or plants with high water content) have a low survival rate or cannot survive at all after recovery. Agapanthus belongs to the tropical Plants, whose embryogenic callus has a high water content, are plant materials that are difficult to preserve, and the cryopreservation technology of Agapanthus has not been reported yet.

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