Preparation method of biological selenium-rich fertilizer
A selenium-enriched fertilizer and biological technology, applied in the preparation of biological selenium-enriched fertilizers, in the field of preparing biological selenium-enriched fertilizers, to achieve the effects of improving multiple effects, obvious application effects, and increasing production
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[0034] 1) Inoculate nitrogen-fixing, phosphorus-dissolving, potassium-dissolving, insect-resistant, disease-resistant, and growth-stimulating strains on the inclined surface of the test tube respectively. Place the slant of the test tube at a constant temperature of 25-32°C for 24-72h.
[0035] 2) Pour the slant of the cultured test tube into sterile water, and scrape the bacterial lawn to prepare a bacterial suspension in a sterile bottle.
[0036] 3) Prepare liquid culture medium, its components are: soybean hydrolyzate 10-30g peptone 4-10g sucrose 16-24g KH 2 PO 4 3~8g MgSO 4 1~6g inorganic selenium 10-100PPM water 1L.
[0037] 4) Steam sterilization, 0.12-0.15Mpa, 121-125°C, 30 minutes.
[0038] 5) Put the strain suspension into the sterilized culture medium.
[0039] 6) Culture at 25-37°C for 36-72 hours.
[0040] 7) Mix the culture fluids obtained from the separate cultures and stir evenly. For the compound ① liquid.
[0041] 8) Activate the strains used for deep...
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