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Diapause maintaining method of Coccinella septempunctata

A seven-star ladybug and diapause technology, applied in animal husbandry and other directions, can solve problems such as inability to apply timely, products cannot be stored for a long time, and the propagation cycle is short, so as to improve the efficiency of pest control, prolong the shelf life of products, and promote large-scale production. multiplication effect

Active Publication Date: 2014-03-26
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in actual production and application, there are often problems such as short multiplication cycle, long-term storage and timely application of products, diapause of adults and influence on multiplication efficiency, etc., which have become technical bottlenecks restricting large-scale production and application of seven-star ladybug. The problem should be based on elucidating the regulatory role of environmental factors (mainly temperature and photoperiod) that affect diapause, so as to master the related technologies for regulating its diapause induction, persistence and release

Method used

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  • Diapause maintaining method of Coccinella septempunctata
  • Diapause maintaining method of Coccinella septempunctata
  • Diapause maintaining method of Coccinella septempunctata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1、 7

[0044] The diapause induction test of embodiment 1, seven-spot ladybird

[0045] 1. Screening test for timing of diapause regulation and manipulation

[0046] Using seven-spot ladybugs (female) at different stages (egg, larva, pupa, and adult) as test materials, at 18°C, refer to the method shown in Table 2 (insect stage-light cycle) for diapause induction, and the induction time for the first eclosion adults was 240h. After the induction, the diapause situation of Coccinosa in each treatment group was observed and the diapause rate was counted. The experiment was repeated 3 times, and the results were averaged. .

[0047] The results are shown in Table 2. The first eclosion adults of the seven-spot ladybug (the adults within 12 hours of eclosion, belonging to the pre-oviposition stage) are the key sensitive insect stages of its diapause, and only the first eclosion adults are in short light (S, photoperiod Under the condition of L10:D14), most of them can enter diapause (...

Embodiment 2、 7

[0075] Embodiment 2, the diapause maintenance test of Coccinella chinensis

[0076] 1. Test method

[0077] (1) Effect of photoperiod: According to the results of diapause induction test in Example 1, under the conditions of temperature 18°C ​​and air relative humidity 70%-80%, the first eclosion adults (within 12 hours) undergo photoperiod L10:D14 , L12:D12, L14:D10 were induced to enter diapause for 240 hours, and the diapause adults were still reared under the original conditions. Observe and record the time when adult diapause is released and eggs start to be laid every day, and about 30 females are observed in each treatment. The experiment was repeated 3 times, and the results were averaged. At the same time, a treatment group in which diapause adults were reared under dark conditions was set up.

[0078] (2) Regulating effect of temperature: According to the results of diapause induction in Example 1, at 24°C and 30°C, the incidence of diapause of adults was lower, a...

Embodiment 3、 7

[0088] Embodiment 3, the diapause release test of Coccinella chinensis

[0089] 1. Test method

[0090] A repetitive test with two factors and two levels each was carried out in an artificial climate chamber (Ningbo Jiangnan RXL-500C). The two photoperiod levels were L10:D14 and L16:D8, and the two temperature levels were 18°C ​​and 24°C. ℃; air relative humidity 70%±10%; light intensity 5000Lx. Adults (41 days old) induced to diapause under the combination of temperature and light at 18°C ​​and L10:D14 were reared under the above four treatment conditions. Observe and record the release of adult diapause every day (those who resume oviposition within the theoretical oviposition period (20 days) will be regarded as individuals who have been released), and the time when oviposition begins. Observe about 30 females in each treatment. The experiment was repeated 3 times, and the results were averaged.

[0091] 2. Test results

[0092] After environmental conditions were chang...

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Abstract

The invention discloses a diapause maintaining and cancellation method of Coccinella septempunctata. The method comprises the following steps: transferring Coccinella septempunctata entering diapause state to a condition of 18 DEG C and lighting period of L10:D14 (or dark) for storage; and after storage, transferring the Coccinella septempunctata to a condition of 24 DEG C and lighting period of L16:D8 to recover development. The method has diapause maintaining time of 114-183 days and diapause canceled in 7.52-8.62 days. The method is of great significance for large-scale propagating Coccinella septempunctata, prolonging shelf period and field prevention / control action time, grasping natural enemy insect development characteristic and occurrence dynamics, improving pest prevention / control efficiency, deepening understanding about natural enemy insect development mechanism, and investigating environment adaptability mechanism and evolution route of insect.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a method for maintaining diapause and relieving diapause of Coccinella chinensis. Background technique [0002] The crop yield loss caused by agricultural diseases and insect pests accounts for 10-15% of the total loss every year in my country. The scientific management of agricultural diseases and insect pests has important economic and social benefits for the implementation of the national food security strategy and the promotion of high agricultural yields. Aphids are the largest pests in agricultural production, and the global losses caused by aphids are nearly 5 billion US dollars each year. At present, chemical insecticides are mainly used to prevent and control aphids. [0003] The use of natural enemies to control aphids can continuously control the damage of aphids through biological control in nature, and maintain the density of aphids below the level of economic harm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
Inventor 张礼生陈红印王伟王孟卿刘晨曦王树英陈长风
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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