Liquid-based cell smear manufacturing and dyeing machine utilizing principles of injection and pipetting

Abstract

The invention relates to a liquid-based cell smear manufacturing and dyeing machine utilizing principles of injection and pipetting, which is characterized by structurally comprising a machine base, a machine body, a worktable, a horizontal slide rail, a moving arm, a horizontal motor moving device, an up-down motor moving device and a microcomputer motion control system, wherein four groups of smear manufacturing and dyeing units and a kit are mounted on the worktable board; and 24 samples can be automatically manufactured into smears and dyed when the machine runs once. The liquid-based cell smear manufacturing and dyeing machine does not need a tedious specific gravity liquid gradient centrifugation step, a micropipe type liquid drawing system of a liquid-based cytology test (LCT) machine, or processes of vacuum negative pressure drawing and positive pressure cell transfer of a microporous filter membrane of a Thinprep cytology test (TCT) machine. Cell samples are drawn, evenly mixed and scattered by using a pipetting injector and sample reagents are transferred by utilizing an accurate quantitative liquid drawing and injecting function of the pipetting injector. Cells in a settling and smear-manufacturing bin are naturally settled on a sticky glass slide by relying on the gravity of the cells and then automatically dyed. The liquid-based cell smear manufacturing and dyeing machine has the advantages that the operation is simple, the cost is relatively low, and high quality liquid-based cytological smears can be rapidly manufactured.

Description

technical field [0001] The invention relates to a liquid-based cytology staining machine using the principle of injection and pipetting, specifically a simple, efficient, high-quality production and high automation liquid-based cytology and dyeing integrated instrument and equipment. In the field of medical clinical pathology, cytology and biological research. Background technique [0002] The traditional manual smear technique has been used almost unchanged for nearly a hundred years. In the 1980s, in the reflection on the traditional manual smear technique, it was noted that there were two main reasons for the occurrence of false negative cases. Firstly, during the smear process, more than 80% of the cells remained on the sampling device and were discarded; secondly, the quality of the smear was poor, and abnormal cells on the slide may overlap each other or be covered by inflammatory cells and red blood cells, making it difficult to identify ; The smear may also affec...

AI Technical Summary

Problems solved by technology

The cell collection and storage process of natural sedimentation LCT is roughly similar to that of TCT. Since the whole film production process uses two specific gravity liquid gradient centrifugation, and then the film production and staining, the entire technical process is tedious and cumbersome.

In addition, there are defects such as high requirements for operator experience and high labor intensity, complex consumable components and high equipment manufacturing costs.

Images