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Bacillus subtilis and application thereof

A technology of Bacillus subtilis and strains, applied in the direction of application, bacteria, fungicides, etc.

Active Publication Date: 2012-10-24
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology prevents fuzzy roots from growing on bananas during their cultivation process by controlling certain factors such as temperature or humidity levels. These technical results help improve quality and yield of crops grown through this methodology.

Problems solved by technology

This patented technical problem addressed in this patents relates to controlling or treating various types of plants infected by fusaria (Fom) without causing harmful side effects on their ecosystem's natural flora.

Method used

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  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1. Isolation and Identification of Bacillus subtilis BLG010

[0032] 1. Isolation of Bacillus subtilis BLG010

[0033] Banana plants in fields with severe Fusarium wilt were selected from banana planting areas in Hainan Province, China, and root soil samples were collected. Air-dry the soil sample, weigh 10g after sieving, put it into a conical flask filled with 90mL sterile water and glass beads, shake it fully, let it stand for 10min, and dilute to 1×10 by gradient concentration method. -3 , 1×10 -4 , 1×10 -5 . Each gradient dilution was heated in a water bath at 80°C for 20 minutes, and 100ul was spread on the nutrient agar (NA) separation medium. Cultivate at 30°C for 2 days, pick and purify representative single colonies according to the characteristics of colony color, shape, dry humidity, transparency, edge, etc. Bacillus was selected and transferred to NA slant medium for preservation as the strain to be tested.

[0034] Antagonist primary screenin...

Embodiment 2

[0041] Example 2. Antagonistic effect of Bacillus subtilis BLG010CGMCC No.5953 on mycelium of Fusarium oxysporum Cuban specialization type 4 (FOC4)

[0042] 1. Inhibitory effect of Bacillus subtilis (Bacillus subtilis) BLG010CGMCC No.5953 fermentation filtrate on pathogenic hyphae

[0043]Add the fermentation filtrate of Bacillus subtilis (Bacillus subtilis) BLG010CGMCC No.5953 (prepared according to the method in step 2 below) to the molten PDA medium and shake well to make a plate. Put a sterile glass slide in the center of the petri dish before pouring the plate, pour the culture medium just below the slide, and connect the 5mm-diameter Fusarium oxysporum Cuban Species 4 (FOC4) block to the slide After culturing at 28°C for 5 days, the slides were taken out and directly placed under a confocal microscope to examine the mycelial morphology of each treatment. Each treatment was repeated 3 times. Microscopic observation found that the fermentation filtrate of Bacillus subtil...

Embodiment 3

[0052] Embodiment 3, potted plant control banana wilt test

[0053] The 3-month-old healthy Brazilian banana seedlings were transplanted into flowerpots filled with diseased soil (containing Fusarium oxysporum race 4 (FOC4)), and subjected to 3 different treatments: Treatment 1: When transplanting, use 200ml concentration of 1×10 8 cfu / mL of Bacillus subtilis (Bacillus subtilis) BLG010CGMCC No.5953 strain bacterial suspension (obtained by suspending the spores of Bacillus subtilis (Bacillus subtilis) BLG010CGMCC No.5953 with water) was watered along the base of bananas, and a total of 150 seedlings were treated; : use 1×10 7 cfu / mL of Bacillus subtilis (Bacillus subtilis) BLG010CGMCC No.5953 bacterial suspension (obtained by suspending the spores of Bacillus subtilis (Bacillus subtilis) BLG010CGMCC No.5953 with water)) along the base of bananas, and treated a total of 100 seedlings; The third is to irrigate clear water as a control, irrigate along the banana base with equal ...

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Abstract

The invention discloses bacillus subtilis and an application thereof. The accession number of the bacillus subtilis BLG010 in the China General Microbiological Culture Collection Center is CGMCC No.5953. The bacillus subtilis BLG010 with number being CGMCC No.5953 can effectively inhibit the growth of fusarium oxysporum f. sp. cubense at low concentration and has great significance on control of banana wilt.

Description

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Claims

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Application Information

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Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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