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Nucleic acid separator for gene detection in treatment on progressive myodystrophy

A technology for muscular dystrophy and gene detection, applied in the field of biomedicine, can solve the problems of tedious and cumbersome, difficult to meet the rapid clinical diagnosis and treatment, etc.

Inactive Publication Date: 2012-10-17
上海奇康生物科技有限公司
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The inventor realized that the above-mentioned detection process has long and cumbersome defects, and it is difficult to meet the needs of clinical rapid diagnosis and treatment, so a more efficient detection method is sought

Method used

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Embodiment Construction

[0013] The magnetic nano-nucleic acid separator used for gene detection in DMD stem cell therapy of the present invention is innovative in the preparation of magnetic nanoparticles, including:

[0014] (1) The synthesized core-shell magnetic nanoparticles are highly dispersed, with a particle size of 30-80nm, suitable for micro-separation, with superparamagnetism and large magnetization, which can ensure efficient separation of samples.

[0015] Further, the particle diameter of the core-shell magnetic nanoparticles is preferably about 50 nm.

[0016] (2) Through the chemical modification method, the required functional groups are modified on the surface of the magnetic nanoparticles, so that the nanoparticles are connected with biomolecules with highly specific recognition functions to form a magnetic nanoparticle-biomolecular complex, thus simplifying the separation Nucleic acid procedure.

[0017] (3) Both the self-designed electromagnetic and permanent magnetic separation...

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Abstract

The invention provides a nucleic acid separator for gene detection in treatment on progressive myodystrophy. The nucleic acid separator comprises core-shell magnetic nano-particles having high dispersity and particle sizes of 30 to 80nm. Through chemical modification, the nucleic acid separator can realize that surfaces of magnetic nano-particles are modified by needed function groups. The nucleic acid separator can realize direct extraction of a genomic DNA from peripheral blood and bone marrow samples, and realize target gene or target nucleic acid detection by a fast reaction system and a magnetic nanotechnology. The invention is suitable for high-flux separation of a gene or a nucleic acid in a clinical stem cell therapy of progressive myodystrophy.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the treatment of progressive muscular dystrophy (DMD), more specifically to a nucleic acid separator for gene detection during the treatment of progressive muscular dystrophy with stem cells. Background technique [0002] Duchenne muscular dystrophy (DMD), also known as progressive pseudohypertrophic muscular dystrophy, is the most common neuromuscular disease in boys. DMD is an X-chromosomal recessive genetic disease characterized by progressive degeneration and necrosis of skeletal muscle. ) deficiency, the main clinical feature is progressive weakness and atrophy of the involved skeletal muscles. Patients typically develop severe disability in their teens and fatal cardiac or respiratory failure in their early to mid-20s. [0003] The standard treatment methods currently used clinically mainly focus on maintaining the general health status of patients and improving the quality of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12Q1/68
Inventor 严整辉
Owner 上海奇康生物科技有限公司
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