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Method for simultaneously determining Pb<2+> and Hg<2+> based on electrochemical DNA biosensor

A biosensor and measurement method technology, applied in the direction of material electrochemical variables, material impedance, etc., can solve the problems of lack of simultaneous determination of metal ions, unfavorable multi-target analysis and detection, etc., to achieve less environmental interference and restrictions, and stability. Good, high-sensitivity detection results

Inactive Publication Date: 2012-10-10
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] As mentioned above, in the current detection of heavy metal ions using electrochemical DNA sensors, there is still a lack of research on the simultaneous determination of two or more metal ions, which is not conducive to the analysis and detection of multiple targets. The sensing and detection technology for detecting multiple ions has practical significance in improving our country's existing detection technology and building an emergency monitoring system for pollution accidents

Method used

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  • Method for simultaneously determining Pb&lt;2+&gt; and Hg&lt;2+&gt; based on electrochemical DNA biosensor
  • Method for simultaneously determining Pb&lt;2+&gt; and Hg&lt;2+&gt; based on electrochemical DNA biosensor
  • Method for simultaneously determining Pb&lt;2+&gt; and Hg&lt;2+&gt; based on electrochemical DNA biosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Hg 2+ separate test

[0028] After fully washing the prepared DNA modified electrode with buffer solution, the K 3 [Fe(CN) 6 ] / K 4 [Fe(CN) 6 ] solution for electrochemical impedance measurement. After the measurement, clean the electrodes, and then place them in different concentrations of H 2+ In solution, use Tris-ClO 4 Electrochemical measurements were performed after rinsing with buffer solution. The changes of membrane capacitance and membrane resistance of DNA-modified electrode before and after treatment are shown in Table 1.

[0029] Table 1 DNA modified electrode and Hg 2+ Impedance data fitting results before and after action

[0030]

Embodiment 2

[0031] Example 2: Pb 2+ separate test

[0032] After fully washing the prepared DNA modified electrode with buffer solution, the K 3 [Fe(CN) 6 ] / K 4 [Fe(CN) 6 ] solution for electrochemical impedance measurement. After the measurement, wash the electrodes, and then place them in different concentrations of Pb 2+ In solution, use Tris-ClO 4 Electrochemical measurements were performed after rinsing with buffer solution. The changes in membrane capacitance and membrane resistance of the DNA-modified electrode before and after the treatment are shown in Table 2.

[0033] Table 2 DNA modified electrode and Ph 2+ Impedance data fitting results before and after action

[0034]

Embodiment 3

[0035] Example 3: Masking of Hg by cysteine 2+ Pb at 2+separate detection

[0036] After fully washing the prepared DNA modified electrode with buffer solution, the K 3 [Fe(CN) 6 ] / K 4 [Fe(CN) 6 ] solution for electrochemical impedance measurement. and then placed in the Hg 2+ +cysteine ​​and Hg 2+ +cysteine+Pb 2+ function in solution, measured in the presence of Hg 2+ Resistive properties of films before and after the presence of masking agents and metal ions. The changes of membrane capacitance and membrane resistance of the DNA-modified electrode before and after the treatment are shown in Table 3.

[0037] Table 3 Cysteine ​​masking Hg 2+ When Pb 2+ Impedance data fitting results before and after interaction with DNA modified electrodes

[0038]

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Abstract

The invention relates to a preparation method and a use of an electrochemical DNA biosensor based on Hg and Pb ion-induced DNA configuration change and belongs to the technical field of analytical chemistry and chemical sensors. The preparation method provided by the invention comprises the following steps of 1, designing a functional zone composed of two parts of DNA sequences respectively used for specifically recognizing Hg ions and Pb ions and 2, assembling the functional zone on the surface of an Au electrode to obtain an electrochemical DNA biosensor. The electrochemical DNA biosensor obtained by the preparation method can be used for detecting a single Hg / Pb system of practical water and can also be used for separately detecting Hg and Pb ions of a mixed system through use of a masking agent. The preparation method of the electrochemical DNA biosensor allows mild conditions, is simple and convenient, and realizes good stability. The electrochemical DNA biosensor has high specificity, high sensitivity and high selectivity, can be operated simply and avoids labeling during detection.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry and chemical sensors, and relates to a preparation method of an electrochemical DNA sensor based on the change of DNA configuration induced by mercury ions and lead ions, and the prepared sensor is applied to an actual water system with two low ion concentrations Simultaneous detection and individual detection of concentration. This method can effectively analyze the pollution characteristics of heavy metal ions in the environment, and build an emergency monitoring system for environmental pollution accidents. Background technique [0002] In recent years, the detection of toxic heavy metal ions using electrochemical sensor methods has become one of the research hotspots. Compared with biosensors based on biocatalysis (enzymes, microorganisms, etc.) and immunological principles, in addition to being able to specifically recognize small molecules, DNA also has a very stable recognitio...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/02
Inventor 刘新会梁刚石柳李晓宏成登苗
Owner BEIJING NORMAL UNIVERSITY
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