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Immunofluorescence test strip component for quickly and quantitatively detecting myocardial creatine kinase isozyme, detection card component comprising same and preparation method

A creatine kinase and immunofluorescence technology, applied in the field of laboratory medicine, can solve the problems of expensive equipment, high missed diagnosis rate, and difficulty in popularization, and achieve the effect of low cost, increased dilution multiple, and good repeatability

Active Publication Date: 2015-01-28
GUANGZHOU HONGQI OPTICAL INSTR TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the shortcomings of the existing CK-MB detection technology, such as the low positive rate of immunosuppressive analysis and the high rate of missed diagnosis, as well as the above-mentioned shortcomings of expensive equipment and difficult popularization of the acridinium ester detection method, according to the characteristics of immunofluorescence technology and the CK-MB antigen-antibody system Features, providing a fast, sensitive and specific test strip for detecting CK-MB

Method used

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  • Immunofluorescence test strip component for quickly and quantitatively detecting myocardial creatine kinase isozyme, detection card component comprising same and preparation method
  • Immunofluorescence test strip component for quickly and quantitatively detecting myocardial creatine kinase isozyme, detection card component comprising same and preparation method
  • Immunofluorescence test strip component for quickly and quantitatively detecting myocardial creatine kinase isozyme, detection card component comprising same and preparation method

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Embodiment 1

[0057] Such as figure 1 As shown, a test strip assembly for rapid quantitative detection of myocardial creatine kinase isoenzyme, including a test strip and a platinum porphyrin-labeled specific antibody used in conjunction with the test strip and independently packaged, the test strip includes a substrate 1 , the water-absorbing pad 2, the coating analysis membrane 3 and the sample pad 6 sequentially bonded on the substrate, the coating analysis membrane 3 is provided with a detection line 4 and a quality control line 5, and the specific antibody coated on the detection line 4 is anti- Myocardial creatine kinase isoenzyme monoclonal antibody, the specific antibody coated by quality control line 5 is rabbit IgG antibody; platinum porphyrin-labeled specific antibody is anti-myocardial creatine kinase isoenzyme monoclonal antibody and anti-rabbit IgG antibody.

[0058] Among them, one side of the bottom liner 1 of the test strip is coated with adhesive or double-sided tape, whic...

Embodiment 2

[0099] In step 2, the preparation method of the detection line coating solution is: 20mM pH7.6 Tris buffer solution (TAPS buffer solution), containing 0.8% methanol, 1% sucrose, 0.6% bovine serum albumin, 10M50 antibody 1 mg / ml. Preparation of quality control line coating solution: 50mM pH7.6 phosphate buffer (PB buffer), containing 0.7% methanol, 0.5% bovine serum albumin, and 0.5mg / ml rabbit IgG. Preparation of the coating film: debug the BIO-DOT film spraying machine, the film liquid volume is 20ul / 40cm, the machine is scribed, the distance between the detection line and the quality control line is 5mm, the scribed line is fine and uniform, and it is placed in a vacuum drying oven at 25°C-37°C for 1.5 Hours, bagged and sealed for later use.

Embodiment 3

[0101] The preparation method of the present embodiment is basically the same as that of the first embodiment, the difference is that:

[0102] In step 3, the anti-CK-MB monoclonal antibody and anti-rabbit IgG antibody were diluted to 1mg / ml with 0.1M sodium bicarbonate solution respectively, and 5ml of antibody solution was taken respectively, and 40mg of fluorescein platinum porphyrin solution was added respectively, and stirred Mix well, incubate at room temperature for 1.5 hours, and mix every 15 minutes. Finally, use G25 gel column to separate and purify, collect the labeled fluorescein-labeled antibody, and use 0.01%-0.5% polyethylene glycol, 1%-5% bovine serum albumin, 5%-20% glycerol, -0.05% surfactant diluted with 0.01M phosphate buffer, sealed in plastic bottles, and stored at 4°C.

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Abstract

An immunofluorescence test strip component for quickly and quantitatively detecting myocardial creatine kinase isozyme comprises a test strip and specific antibodies with platinum-porphyrin marks, the specific antibodies are used with the test strip and are independently packed, the test strip comprises a bottom liner, an absorbent pad, an envelope analysis membrane and a sample pad, the absorbent pad, the envelope analysis membrane and the sample pad are sequentially jointed on the bottom liner, a detection line and a quality control line are disposed on the envelope analysis membrane, the specific antibody enveloped by the detection line is a monoclonal antibody resisting the myocardial creatine kinase isozyme, the specific antibody enveloped by the quality control line is a rabbit IgG (intravenous gamma globulin) antibody, and accordingly the specific bodies with the platinum-porphyrin marks are the monoclonal antibody resisting the myocardial creatine kinase isozyme and the antibody resisting the rabbit IgG. The invention further discloses a preparation method for the detection test strip component, a detection card comprising the test strip component and a preparation method of the detection card. The detection test strip component can simply, quickly and sensibly detect the level of the myocardial creatine kinase isozyme in a human body, and is used for diagnosing acute myocardial infarction.

Description

technical field [0001] The invention relates to the field of laboratory medicine, in particular to an immunofluorescence test strip component for rapid quantitative detection of myocardial creatine kinase isoenzyme, a detection card component and a preparation method thereof. Background technique [0002] Creatine kinase (CK) is a dimer consisting of two subunits, M and B, to form CK–MM, CK-MB and CK–BB isozymes. CK–BB is found in brain tissue, and CK–MM and CK-MB are found in various muscle tissues. In different tissues, the ratio of CK isoenzyme is different. In skeletal muscle, 98%-99% is CK-MM, and 1%-2% is CK-MB; in myocardium, about 80% is also CK-MM, but CK-MB accounts for 15% to 25% of total myocardial CK. [0003] The most important significance of serum CK-MB determination lies in the diagnosis of acute myocardial infarction. 4 to 6 hours after the onset of chest pain in acute myocardial infarction, the serum CK-MB activity of patients begins to increase before ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/573
Inventor 罗琪谢爱武梁万兴李必松
Owner GUANGZHOU HONGQI OPTICAL INSTR TECH
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