Gene recombinant human active basic fibroblast growth factor fusion protein, preparation method thereof and application thereof

Abstract

The invention provides a gene recombinant human active basic fibroblast growth factor fusion protein, wherein the whole length of the fusion protein is 762 amino acids, the nitrogen end is I type human-like collagen 500-1099 peptide and jointly has 600 amino acids, the carbon end is human basic fibroblast growth factor and jointly has 154 amino acids, and two peptides are connected with each other by glutamic acid, phenylalanine and 6 amino acids-containing flexible peptide. A preparation method of the gene recombinant human active basic fibroblast growth factor fusion protein comprises the following steps of: constructing a gene recombinant human active basic fibroblast growth factor fusion protein expression vector, electrically transforming pichia pastoris, screening multi-copy inserting recon, fermenting the gene recombinant human active basic fibroblast growth factor fusion protein, and purifying the gene recombinant human active basic fibroblast growth factor fusion protein. Thegene recombinant human active basic fibroblast growth factor fusion protein is used for preparing the cosmetics.

Description

technical field [0001] The invention relates to a gene recombination fusion protein, in particular to a basic fibroblast growth factor and collagen fusion protein. Background technique [0002] Basic fibroblast growth factor (Basic fibroblast growth factor, bFGF), a member of the fibroblast growth factor family, was originally extracted from the bovine pituitary gland, because it can promote fibroblast proliferation and isoelectric The point is 9.6, so it is called basic fibroblast growth factor. Basic fibroblast growth factor is composed of 155 amino acids with a molecular weight of about 18kD. It is unstable to heat and easily degraded by proteases. It widely exists in cells derived from mesoderm and neuroectoderm and various tumor cells. A polypeptide growth factor that can promote the proliferation of mesoderm and neuroectoderm cells. In terms of biological evolution, basic fibroblast growth factor is highly conserved. There are only two amino acid differences between ...

AI Technical Summary

Problems solved by technology

In recent years, recombinant basic fibroblast growth factor has also been widely used in the clinical treatment of wounds, ulcers and nervous system diseases, but the expression level is generally low, making its production cost relatively high

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