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Cell culture device capable of generating multiple magnetic fields and culture method

A technology of cell culture and magnetic field, which is applied in tissue cell/virus culture devices, stress-stimulated microbial growth methods, biochemical cleaning devices, etc. It can solve the problems of powerlessness and device inability to perform cell culture, and achieve reliable equipment and methods Effect

Active Publication Date: 2013-11-06
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The devices of the above inventions have their own limitations. For example, the devices that generate complex magnetic fields cannot perform cell culture, while the devices that can perform cell culture have only a single static magnetic field.
These devices appear powerless when analyzing the effects of alternating and rotating magnetic fields on cells

Method used

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  • Cell culture device capable of generating multiple magnetic fields and culture method
  • Cell culture device capable of generating multiple magnetic fields and culture method
  • Cell culture device capable of generating multiple magnetic fields and culture method

Examples

Experimental program
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Effect test

Embodiment 1

[0067] A cell culture device capable of generating various magnetic fields, the device is a box, and a heating tube 30 and a CO are arranged on the side wall of the box 2 The access hole is provided with a cooling pipe 32 on its opposite side wall, and a temperature sensor 34 and CO are installed on the bottom surface. 2 The sensor 35 is equipped with a coil 31 in the middle of the box. The coil 31 includes three sets of Helmholtz coils placed orthogonally in space. Each set of coils has two taps, which are respectively connected to the control circuit 36 ​​for access to excitation. Current, the axes of each group of coils are respectively defined as X, Y, and Z axes, and the intersection point is the origin O, and the stage 33, CO 2 The generating part 29 feeds CO into the culture device through the pipeline and the hole on the side wall of the box.2 , the computer 1 is connected to the temperature sensor 34, CO respectively via the control circuit 36 2 The sensor 35, the te...

Embodiment 2

[0074] A cell culture device capable of generating various magnetic fields, the device is a box, and a heating tube 30 and a CO are arranged on the side wall of the box 2 The access hole is provided with a cooling pipe 32 on its opposite side wall, and a temperature sensor 34 and CO are installed on the bottom surface. 2 The sensor 35 is equipped with a coil 31 in the middle of the box. The coil 31 includes three sets of Helmholtz coils placed orthogonally in space. Each set of coils has two taps, which are respectively connected to the control circuit 36 ​​for access to excitation. Current, the axes of each group of coils are respectively defined as X, Y, and Z axes, and the intersection point is the origin O, and the stage 33, CO 2 The generating part 29 feeds CO into the culture device through the pipeline and the hole on the side wall of the box. 2 , the computer 1 is connected to the temperature sensor 34, CO respectively via the control circuit 36 2 The sensor 35, the ...

Embodiment 3

[0081] A cell culture device capable of generating various magnetic fields, the device is a box, and a heating tube 30 and a CO are arranged on the side wall of the box 2 The access hole is provided with a cooling pipe 32 on its opposite side wall, and a temperature sensor 34 and CO are installed on the bottom surface. 2 The sensor 35 is equipped with a coil 31 in the middle of the box. The coil 31 includes three sets of Helmholtz coils placed orthogonally in space. Each set of coils has two taps, which are respectively connected to the control circuit 36 ​​for access to excitation. Current, the axes of each group of coils are respectively defined as X, Y, and Z axes, and the intersection point is the origin O, and the stage 33, CO 2 The generating part 29 feeds CO into the culture device through the pipeline and the hole on the side wall of the box. 2 , the computer 1 is connected to the temperature sensor 34, CO respectively via the control circuit 36 2 The sensor 35, the ...

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Abstract

The invention relates to a cell culture device capable of generating multiple magnetic fields. Through three groups of orthogonal Helmholtz coils and different excitation modes thereof, a single axial steady magnetic field, a single axial alternating magnetic field, a planar rotating magnetic field and a spatial rotating magnetic field can be generated. Meanwhile, through the means of digital control and the like, control on the magnetic induction density, magnetic field frequency, temperature and CO2 concentration in the device can be realized to satisfy the conditions for the cell growth and survival. The steady, alternating, planer and spatial rotating magnetic fields can be generated, and the adjustment of the field intensity, magnetic field frequency and action time is realized through computer control; the limit that the traditional device can generate a single magnetic field is compensated; and reliable equipment and method are provided for exploring the influence and response change of different magnetic fields on the survival of the tumor cell cultured in vitro, the molecule function, the intracellular signal transduction network and cytoskeleton reconstruction and for clarifying the action mechanism of each magnetic field.

Description

Technical field: [0001] The invention relates to a cell culture device and method, in particular to a cell culture device and method capable of generating various magnetic fields for culturing cells in the device and analyzing their survival state and signal pathway. Background technique: [0002] The magnetic field is divided into a steady magnetic field and an alternating magnetic field. Steady magnetic field refers to a magnetic field whose size and direction do not change with time generated by a constant current. When the coil is supplied with an alternating current, an alternating magnetic field is generated. Rotating magnetic field is a special form of alternating magnetic field. It can pass through a spatially symmetrical three-phase load and a time-symmetrical three-phase alternating current, and finally cause the magnetic induction vector to rotate at a fixed frequency in space. [0003] Many existing studies point out that the use of magnetic field to treat tumo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/42C12M1/38C12M1/36C12M1/34C12N5/00C12N13/00
CPCC12M35/06C12M41/12
Inventor 林婷婷史文龙
Owner JILIN UNIV
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