Pesticide composition containing orysastrobin
A technology of pesticide composition, oryzastrobin, which is applied in the field of pesticide compounding, to achieve significant synergistic effect, strong drug effect and good long-lasting effect
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Embodiment 1
[0041] Toxicity determination of oryzastrobin combined with any of econazole, hexaconazole and triconazole against rice blast fungus in laboratory
[0042] Test object: rice blast fungus collected from the field
[0043] Test method: refer to "People's Republic of China Agricultural Industry Standard NY / T1154.7-2006", pot method. Select rice seedlings at the three-leaf stage with consistent growth potential, 2 seedlings per pot, and 5 pots of rice seedlings for each treatment. Cultivate Magnaporthe grisea on tomato oat agar medium, wash the spores with sterile water after spore production, and make 1×10 5 spores / mL suspension, sprayed evenly on the rice seedlings to be tested, and covered with a black plastic bag to moisturize and cultivate for 24 hours after inoculation. 24 hours after inoculation, carry out medicament treatment, set 5 concentration gradients for each medicament, and spray at 50PSI pressure with a Potter spray tower, about 5mL per pot. After spraying, the ...
Embodiment 2
[0062] Toxicity determination of orysastrobin combined with any of econazole, hexaconazole and triaconazole against rice sheath blight
[0063] Test object: Rice sheath blight fungus collected from the field
[0064] Test method: refer to "People's Republic of China Agricultural Industry Standard NY / T1154.7-2006", broad bean detached leaf method. Rice sheath blight fungus is cultivated with PDA medium, and when the bacterial colony just covers the petri dish, a puncher with an inner diameter of 5 mm is used to punch holes from the edge, and the resulting mycelium block is used as an inoculum. Select broad bean leaves with the same leaf position, 10 leaves for each treatment, soak in the prepared medicinal solution for 10 minutes, take it out to dry, put it in a petri dish covered with moisturizing paper, and set 5 concentration gradients for each medicinal solution. Finally, the mycelium block was placed upside down in the center of the leaves, and cultured in a constant temp...
Embodiment 3
[0077] Biological Assay Example 3: Indoor Toxicity Determination of Oryzastrobin and Econazole, Hexaconazole, and Triconazole Compounded to Rice Aspergillus
[0078] Test object: rice smut fungus collected from the field
[0079] Test method: refer to "People's Republic of China Agricultural Industry Standard NY / T1154.7-2006", mycelium growth rate method. The rice smut fungus is cultivated with PDA medium. When the bacterial colony is just full of the petri dish, a puncher with an inner diameter of 7 mm is used to punch holes from the edge, and the resulting mycelium block is used as an inoculum. Take 5ml of prepared medicine solution and 75ml of sterilized culture medium and mix evenly to make 4 medicine-containing liquid plates, and mix equal amount of sterile water and culture medium as a control. The mycelium block was placed upside down and placed in the center of the plate, and cultured in a 28°C incubator. After 7 days, the colony diameter was measured by the cross me...
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