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Genes related to stress resistance and application to improving stress resistance of plant to environment

An abiotic stress, gene technology, applied in the application, plant products, genetic engineering and other directions, can solve the problems of unsustainable effect and high cost, and achieve the goal of improving plant salt tolerance, abiotic stress tolerance, and abiotic stress tolerance. Effects of biotic stress resistance

Inactive Publication Date: 2012-08-01
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are more or less insurmountable shortcomings in many improvement measures. For example, using fresh water to drain salt will drain other nutrients in the soil together, and the rest have shortcomings such as unsustainable effects or expensive costs. Therefore, improving the drought resistance of plants , Salt tolerance, that is, to modify the genes of the plants themselves, is a fundamental and effective method to improve crop yields and arable land utilization

Method used

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  • Genes related to stress resistance and application to improving stress resistance of plant to environment
  • Genes related to stress resistance and application to improving stress resistance of plant to environment
  • Genes related to stress resistance and application to improving stress resistance of plant to environment

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Embodiment 1

[0041] The cultivation of embodiment 1 transfer Mn-SOD gene, Cu / Zn-SOD gene or Fe / Mn-SOD gene tobacco

[0042] 1. Construction of transgene expression vector

[0043] 1. Acquisition of Mn-SOD gene, Cu / Zn-SOD gene and Fe / Mn-SOD gene

[0044] The present invention clones three SOD genes from a strain of Geobacillus sp. The primers used in PCR are: the upstream primer C of Mn-SOD gene GAGCTC ATGCCATTTGAATTGCCAGC, introduce restriction site SacI, downstream primer C GGATCC TTACTTCGCTTTCGCTTCGC, introducing restriction site BamHI; upstream primer C of Cu / Zn-SOD gene GAGCTC ATGCGAAAAACGTACGGTGTG, the introduction of enzyme cutting site SacI, downstream primer C GGATCC TCACGACGACTTGATTTCCC, introducing restriction site BamH I; upstream primer C of Fe / Mn-SOD gene GGATCC ATGCGTGGGGCAAGCAC, introduce restriction site BamHI, downstream primer C TCTAGA TTAAAACGGCTGCCAACG, introduce restriction site XbaI. The PCR amplification system was 50 μL, and 1 μL of genomic DNA was used...

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Abstract

The invention discloses genes related to stress resistance and application to improving stress resistance of a plant to environment. According to the invention, three SOD (Super Oxygen Dehydrogenises) genes, i.e., Mn-SOD gene, Cu / Zn-SOD gene and Fe / Mn-SOD gene, are separated from geobacillus sp., wherein the nucleotide sequences of the three genes are shown as SEQ ID No. 1, SEQ ID No. 2 or SEQ ID No. 3 respectively. The invention further provides application of the SOD genes to improving stress resistance of the plant to environment. The application comprises the following steps of: constructing a plant expression vector containing the SOD genes; transforming the constructed plant expression vector into planet cells or plant tissues; and regenerating to obtain transgenic plant strain with improved stress resistance to non-living things.

Description

technical field [0001] The present invention relates to stress resistance-related genes and applications thereof, in particular to three SOD genes isolated and cloned from Geobacillus sp and their application in improving plant resistance to abiotic stress, belonging to plant stress resistance field of molecular biology. Background technique [0002] Abiotic stresses such as drought and salinity have seriously affected plant growth and crop yield, and the damage to agricultural production is worldwide (Boyer JS, Science, 1982; 218: 443-448). Abiotic stress is the main cause of crop yield reduction, which can lead to an average crop yield reduction of 50% to 80%. Plants have a variety of molecular, cellular, physiological and biochemical mechanisms to resist and adapt to various abiotic stresses. Although the genes and molecular mechanisms of plant resistance to abiotic stress have not been fully elucidated so far, they can be resisted from many aspects such as genetics, cel...

Claims

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Application Information

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IPC IPC(8): C12N15/53C12N15/82A01H5/00
Inventor 李轶女张志芳潘沈元姚冉叶兴国王金辉杨灵王国增沈桂芳
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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