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Recovery culturable viable but non culturable (VBNC) arthrobacterium DSC4 strain and recovering method and application thereof

A technology of Arthrobacter and bacterial strains, applied in the field of environmental protection, biotechnology and agriculture, to achieve obvious denitrification and denitrification effects

Active Publication Date: 2014-12-10
郎溪品旭科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no public reports on the technology of isolating the nitrifying bacteria in the VBNC state in the sewage treatment system and the recovery of the cultivable VBNC state nitrifying bacteria in China.

Method used

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  • Recovery culturable viable but non culturable (VBNC) arthrobacterium DSC4 strain and recovering method and application thereof
  • Recovery culturable viable but non culturable (VBNC) arthrobacterium DSC4 strain and recovering method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1 From the sewage biological treatment system, the preservation of the VBNC Arthrobacter DSC4 strain was obtained by screening and cultivating the technology of resuscitation. The cultivable VBNC Arthrobacter DSC4 strain was isolated from a certain sewage biological treatment system, and there were more than 90% of the strain in the system Generally, it is difficult to obtain by traditional isolation methods, and the viable non-culturable (VBNC) is similar to the dormant bacteria. The Arthrobacter DSC4 strain in the VBNC state was screened by using the resuscitated culture technology. The preservation number of the strain is CCTCC No: M2011406, the preservation unit is: China Center for Type Culture Collection, the preservation address is: Wuhan University, Wuhan, China, and the preservation date is: November 21, 2011. The classification is named: Arthrobacter DSC4 Arthrobacter sp.DSC4, and this strain generally needs to be stored in ultra-low temperature freezin...

Embodiment 2

[0033] Example 2 The cultivating and identifying method for the denitrification and denitrification effect of the cultivable Arthrobacter VBNC DSC4 strain on the nitrate matrix, including the following steps: ① Take the cultured Arthrobacter VBNC Arthrobacter DSC4 strain and store it under aseptic conditions. , pick a small amount of strains and transfer them to NYGR medium, and activate them at 30°C for 2-4 days; Introduce 2% of the pre-culture bacteria solution into the Giltay medium, and culture it on a shaker at 30°C and 120 rpm for 2 days. Observe the gas production and color changes of the medium, and measure the ammonia nitrogen and nitrite nitrogen in the culture medium. , nitrate nitrogen and total nitrogen.

[0034] Ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and total nitrogen were respectively used by Nessler colorimetry, ultraviolet spectrophotometry, national standard water quality total nitrogen determination method (GB11894-89 determination of water qu...

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Abstract

The invention belongs to the fields of agricultural biotechnology and environmental protection and relates to a 'viable but non culturable (VBNC)' arthrobacterium strain which is obtained through separation by using recovery culturable technology, a recovering method of the strain and application of the strain. The preservation number of the strain is CCTCC No: M2011406; the preservation organization is China Center for Type Culture Collection; the preservation address is Wuhan University, Wuhan Province, China; and the preservation date is 25th Nov. 2011. The viable but non culturable (VBNC) arthrobacterium DSC4 can turn the color of the reaction liquid of a Giltay culture medium to dark blue from green and generate gas. At the same time, the conversion rate of nitrate nitrogen is detected to be 54.7 percent, the total nitrogen depletion rate is 52.9 percent, and residue of ammoniacal nitrogen and nitrite nitrogen is not detected. The strain has an obvious denitrification effect.

Description

technical field [0001] The invention belongs to the fields of biotechnology agriculture and environmental protection, and relates to a "viable non-culturable (VBNC)" Arthrobacter sp. methods and applications. Background technique [0002] The biological treatment of ammonia nitrogen-containing sewage is an extremely important technical measure in today's agriculture and environmental protection industries. Among them, the oxidation of ammonia nitrogen, the oxidation of nitrite nitrogen, the reduction of nitrate nitrogen and the denitrification of key technical links need more With the participation of various microorganisms, it degrades and transforms inorganic and organic nitrogen. Generally, traditional separable single-function nitrosifying bacteria such as Nitrosomonas and Nitrosococcus species, nitrifying bacteria such as Nitrobacter and Nitrococcus species, denitrifying and denitrifying bacteria such as Micrococcus denitrificans are autotrophic bacteria, and nitrifica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/06
Inventor 丁林贤陈建荣金夷林冬珍
Owner 郎溪品旭科技发展有限公司
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