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Extraction method of aristolochic acid A in swelling dispersing and pain relieving tincture

An extraction method and technology of aristolochic acid, applied in the field of medicine, can solve the problems of affecting the content determination effect and having many impurities in the extract

Active Publication Date: 2012-07-18
广西壮族自治区花红药业集团股份公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0002] Anti-swelling and pain-relieving tincture is the exclusive product of Huahong Pharmaceutical Co., Ltd. in Guangxi Zhuang Autonomous Region. "Quality Control Method for Aristolochic Acid A in Asarum Medicinal Materials and Xiaozhongzhitong Tincture" discloses a method for extracting aristolochic acid A in Xiaozhongzhitong tincture. When extracting nanogram-level aristolochic acid , there is a problem that there are many impurities in the extract, which affect the effect of content determination

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Test product: using anti-swelling and analgesic tincture, a commercially available product, produced by Huahong Pharmaceutical Co., Ltd. of Guangxi Zhuang Autonomous Region, batch number: 20080303

[0024] The specific operation of the inspection of the swelling and pain-relieving tincture is as follows:

[0025] 1. Chromatographic conditions:

[0026] Chromatographic column: silica gel column bonded with octadecylsilane;

[0027] The mobile phase is composed of: 0.1% ammonium carbonate solution-acetonitrile with a mass percentage of 10% hydrochloric acid solution to adjust the pH to 7, wherein the volume ratio of the solution to acetonitrile is 70:30;

[0028] Flow rate: 0.8ml / min. ;

[0029] Detection wavelength: 240nm.

[0030] 2. Preparation of the reference solution: Accurately weigh an appropriate amount of aristolochic acid A, add methanol to make a solution containing 1 μg of aristolochic acid A per milliliter.

[0031] 3. Preparation of the test solution: ...

Embodiment 2

[0035] Test product: using anti-swelling and analgesic tincture, a commercially available product, produced by Huahong Pharmaceutical Co., Ltd. of Guangxi Zhuang Autonomous Region, batch number: 20090201

[0036] The specific operation of the inspection of the swelling and pain-relieving tincture is as follows:

[0037] 1. Chromatographic conditions:

[0038] Chromatographic column: silica gel column bonded with octadecylsilane;

[0039] The mobile phase is composed of: 0.1% ammonium carbonate solution-acetonitrile with a mass percentage of 10% hydrochloric acid solution to adjust the pH to 7, wherein the volume ratio of the solution to acetonitrile is 80:20;

[0040] Flow rate: 1ml / min. ;

[0041] Detection wavelength: 250nm.

[0042] 2. Preparation of the reference solution: Accurately weigh an appropriate amount of aristolochic acid A, add methanol to make a solution containing 2 μg of aristolochic acid A per milliliter.

[0043] 3. Preparation of the test solution: Pr...

Embodiment 3

[0047] Test product: using anti-swelling and pain-relieving tincture, a commercially available product, produced by Huahong Pharmaceutical Co., Ltd. of Guangxi Zhuang Autonomous Region, batch number: 20100804

[0048] The specific operation of the inspection of the swelling and pain-relieving tincture is as follows:

[0049] 1. Chromatographic conditions:

[0050] Chromatographic column: silica gel column bonded with octadecylsilane;

[0051] The mobile phase is composed of: 0.1% ammonium carbonate solution-acetonitrile with a mass percentage of 10% hydrochloric acid solution to adjust the pH to 7, wherein the volume ratio of the solution to acetonitrile is 90:10;

[0052] Flow rate: 1.5ml / min. ;

[0053] Detection wavelength: 260nm.

[0054] 2. Preparation of the reference solution: Accurately weigh an appropriate amount of aristolochic acid A, add methanol to make a solution containing 5 μg of aristolochic acid A per milliliter.

[0055] 3. Preparation of the test solutio...

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Abstract

The invention relates to an extraction method of aristolochic acid A in a swelling dispersing and pain relieving tincture, and aims to solve the extraction problem of aristolochic acid A in the swelling dispersing and pain relieving tincture and meet the requirements for determining the content of aristolochic acid A in the swelling dispersing and pain relieving tincture. The method provided in the invention has the advantages of high extraction efficiency, reliable accuracy and very high practical value.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a method for extracting aristolochic acid A from swelling and pain-relieving tincture. Background technique [0002] Anti-swelling and pain-relieving tincture is the exclusive product of Huahong Pharmaceutical Co., Ltd. in Guangxi Zhuang Autonomous Region. "Quality Control Method for Aristolochic Acid A in Asarum Medicinal Materials and Xiaozhongzhitong Tincture" discloses a method for extracting aristolochic acid A in Xiaozhongzhitong tincture. When extracting nanogram-level aristolochic acid , there are many impurities in the extract, which affects the effect of content determination. [0003] Therefore, it is necessary to conduct further research on the extraction method to find a more efficient method for extracting aristolochic acid A in the anti-tumor and pain-relieving tincture. Contents of the invention [0004] The invention provides a method for extracting aristolochic acid ...

Claims

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Application Information

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IPC IPC(8): C07D317/70
Inventor 唐秀玲
Owner 广西壮族自治区花红药业集团股份公司
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