High-efficiency rapid rabbit-eye blueberry breeding method through tissue culture

Abstract

The invention belongs to the technical field of plant tissue culture and particularly relates to a high-efficiency rapid rabbit-eye blueberry breeding method through tissue culture. Primary inducing culture medium used in the method is improved WPM+Zt 1.0-1.5mg / L, successive multiplication culture medium is improved WPM+Zt 0.5-0.8mg / L, and rooting culture medium is 1 / 2 improved WPM+IAA 0.1-0.2mg / L+AC 0.5g / L. The method comprises the steps of: dipping the stem bases of successively multiplied aseptic blueberry seedlings in IBA sterile solution with concentration being 100-500mg / L for 5-15 seconds, inoculating the stem bases in the blueberry rooting culture medium, moving a rooting tissue culture seedling culture bottle from a culture room to a plastic greenhouse for training seedlings for 4-7 days after rooting, uncovering the plastic greenhouse and training the seedlings for 3-5 days before transplanting, transplanting the seedlings in culture medium in a cell tray, laying a plastic film to preserve moisture for 5-7 days, ventilating twice a day for 5-15 minutes per time during moisture preservation and fully removing the plastic film after 7 days. The high-efficiency rapid rabbit-eye blueberry breeding method through tissue culture has the advantages that not only is the efficiency high, but also the cost is lower and the mass production is facilitated.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a high-efficiency tissue culture and rapid propagation method of rabbit eye blueberry. Background technique [0002] Blueberry (Vaccinium spp.) belongs to Ericaceae (Ericaceae) Vaccinium (Vaccinium) perennial deciduous or evergreen shrub fruit trees. Blueberry fruit has extremely high nutritional health value and economic value, and its nutritional value is much higher than other fruits. It is often known as "the king of berries in the world"; It has been identified as one of the five health foods for human beings by the Food and Agriculture Organization of the United Nations (FAO). Due to the unique flavor and high nutritional and health value of blueberry, its fruit and products are popular all over the world, and the supply exceeds demand, and the price is expensive in the international market. Although the scale of blueberry planting in the world is...

AI Technical Summary

Problems solved by technology

In recent years, the planting area of ​​blueberries in my country has grown rapidly, but according to the development law of blueberries in the world and the forecast of the development of China's blueberry industry, the moderate planting scale of blueberries in my country should be 20,000 ha 2 Above, therefore, there is a lot of room for development

[0003] Due to the high nutritional value and unique health benefits of blueberries, the development momentum is strong, and common propagation methods such as cuttings and root divisions have been difficult to meet the growing market demand for blueberry seedlings.

For this reason, studies on the rapid propagation of blueberry tissue culture have been carried out at home and abroad. Existing studies believe that WPM basic medium is the best for blueberry tissue culture, and higher blueberry tissue can be obtained after adding a certain concentration of plant growth regulator zeatin. Cultivation multiplication factor; however, blueberries are slower to root in test tubes and have a lower rooting rate than tissue cultures of plants such as apples and strawberries

Generally, apples and strawberries can complete the rooting process within 20 days after inoculation, and the rooting rate reaches more than 95%, while the rooting rate of blueberries is less than 10% after 20 days of cultivation in the medium with 1 / 2WPM added IBA, and the rooting rate after 40 days of cultivation is also 10%. Only 30% to 70%, some varieties can take root at the shoot base after being cultivated in the culture medium for 60 days or longer, or grow aerial roots outside the culture medium, so it is difficult to establish an efficient blueberry tissue culture rapid propagation system, thereby Unable to achieve large-scale factory production of blueberries

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