Culture method for rapidly increasing beta-glucan content in coccolithophores

A culture method and technology of coccolithophores, which is applied in the field of rapidly increasing the content of β-glucan in coccolithophores, can solve the problems of uncultivated method reports, etc., and achieve the improvement of β-glucan content, rapid proliferation, The effect of high accumulation rate

Inactive Publication Date: 2012-06-27
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no public report on the culture of microalgae that increases the content of β-glucan, and there is no report on the cultivation method for increasing the content of β-glucan in coccolithophores. Usually, the β-glucan of mature coccolithophores is About 20.36pg / cell (dry weight basis), the yield (weight of β-glucan pure sugar / weight of algal powder) is about 0.31%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] A rapid increase in coccolithophores β- The culture method of dextran content, with 2.0×10 5 cell / ml inoculation density Inoculate coccolithophores seedlings in seawater culture solution with a salinity of 25-26‰, cultivate them at a temperature of 21-23°C and a light intensity of 2500-3000 Lux. 8 hours before algal maturation, add sodium dihydrogen phosphate solution with a concentration of 5g / L to the seawater culture solution, the volume ratio of seawater culture solution and sodium dihydrogen phosphate solution is 1000:1, and cultivate for 8 hours until the coccolithophores mature Harvesting the body, the coccolithophores obtained in this way mature in the body β- The content of glucan is about 22.54pg / cell (dry weight basis), the yield (weight of β-glucan pure sugar / weight of algal powder) is about 0.348%, and the purity is 94.21%.

Embodiment 2

[0014] A rapid increase in coccolithophores β- A culture method for glucan content, a method for rapidly increasing coccolithophores β- The culture method of dextran content, with 1.9×10 5 cell / ml inoculation density Inoculate coccolithophores seedlings in seawater culture solution with a salinity of 26-28‰, cultivate them at a temperature of 15-20°C and a light intensity of 3000-5000 Lux. 5 hours before the maturation of algae, add sodium dihydrogen phosphate solution with a concentration of 5g / L to the seawater culture solution. The volume ratio of seawater culture solution to sodium dihydrogen phosphate solution is 1000: 1.2, and cultivate for 5 hours until the mature coccolithophores Harvesting, the mature coccolithophores obtained in this way β- The glucan is about 22.26pg / cell (dry weight basis), the yield (weight of β-glucan pure sugar / weight of algal powder) is about 0.343%, and the purity is 93.94%.

Embodiment 3

[0016] A rapid increase in coccolithophores β- A culture method for glucan content, a method for rapidly increasing coccolithophores β- The culture method of dextran content, with 2.1×10 5 cell / ml inoculation density Inoculate coccolithophores seedlings in seawater culture solution with a salinity of 28-30‰, cultivate them at a temperature of 23-25°C and a light intensity of 2000-3000 Lux. 10 hours before the algae mature, add a sodium dihydrogen phosphate solution with a concentration of 5g / L to the seawater culture solution, the volume ratio of the seawater culture solution to the sodium dihydrogen phosphate solution is 1000: 0.8, and cultivate for 10 hours until the coccolithophores mature body harvesting, the mature coccolithophores obtained in this way β- The dextran is about 22.32pg / cell (dry weight basis), and the purity is 93.43%.

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Abstract

The invention discloses a culture method for rapidly increasing beta-glucan content in coccolithophores, which comprises the following steps: inoculating coccolithophore seeds in a seawater culture solution of salinity 25-30 per mill, culturing in conditions of a temperature 15-25 DEG C and intensity of illumination 2,000-5,000Lux, and adding sodium dihydrogen phosphate of concentration 5g / L in the culture solution before coccolithophore mature bodies are collected, wherein the volume ratio of the seawater culture solution and sodium dihydrogen phosphate solution is 1,000:(0.8-1.2). In such a way, within the last 5-10 hours before collection, i.e. the period in which the cell density of coccolithophore mature bodies is the highest, the addition of sodium dihydrogen phosphate enables rapid metabolism of coccolithophore mature bodies and rapid increase of beta-glucan, resulting in a high cumulative yield, and because the coccolithophore mature bodies are collected after 5-10 hours, the consumption is less, the beta-glucan content in the coccolithophore mature bodies is high, up to over 22 pg / cell, the purity is up to over 93%, and the bioactivity is high.

Description

technical field [0001] The invention relates to a method for cultivating coccolithophores, in particular to a method for rapidly increasing the content of beta-glucan in coccolithophores. Background technique [0002] β-glucan is metabolized and accumulated in organisms such as bacteria, fungi, higher plants, and microalgae. It has functions such as anti-tumor, anti-infection, lowering cholesterol, enhancing defense system, phagocytosis, and immune regulation. It is an animal bait An important raw material for additives, health food additives and active immune preparations. The yield, purity and activity of β-glucan extracted from different biological sources are also different. Therefore, people are still working on screening β-glucans from different biological sources with stable high content and high biological activity . Marine microalgae (such as Chaetoceros, Chrysophyte 3011, and coccolithophores) are an important part of marine biological resources. Due to the advan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12R1/89
Inventor 周成旭田甜严小军刘宝宁陈海敏骆其君
Owner NINGBO UNIV
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