Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cotton acetylcysteine (NAC) transcription factor NST sub-family and application thereof

A transcription factor and subfamily technology, applied in the field of genetic engineering, can solve problems such as the undiscovered cotton NAC transcription factor NST subfamily

Inactive Publication Date: 2012-06-13
BEIJING NORMAL UNIVERSITY
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the NST subfamily of cotton NAC transcription factors

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cotton acetylcysteine (NAC) transcription factor NST sub-family and application thereof
  • Cotton acetylcysteine (NAC) transcription factor NST sub-family and application thereof
  • Cotton acetylcysteine (NAC) transcription factor NST sub-family and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] Example Acquisition and function of three genes GhNST1, GhNST2 and GhNST3 of the NST subfamily of cotton NAC transcription factor

[0027] 1 Materials and methods

[0028] 1.1 Plant materials, strains, plasmid vectors

[0029] 1.1.1 Plant materials

[0030] Henan Anyang Upland Cotton (Gossypium hirsutum) Kezi 304 is planted in the Biological Park of the College of Life Sciences, Beijing Normal University, under conventional field management (planted in April, and harvested from August to September). The cotton bolls are listed on the day of flowering, which is regarded as the 0 day of flowering. Take cotton bolls with different developmental stages of 0d, 3d, 5d, 10d, 15d and 20d, and quickly strip the fibers and ovules and place them in liquid nitrogen. The young leaves were taken, the flowers were quickly frozen in liquid nitrogen, and the roots and stems were taken from 15-d seedlings cultivated in the greenhouse. Store at -80℃ for later use.

[0031] Colombian wild-type A...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention utilizes bioinformatics and 3'-race to successfully clone cotton fiber blooming for 10 days and obtain three full-length genomes. The three full-length genomes obtained through bioinformatic comparison and cladogram analysis belong to an acetylcysteine (NAC) NST sub-family and are named as GhNST1, GhNST2 and GhNST3 respectively. Amino acid sequences of the three full-length genomes are shown as Seq ID No. 1-3. By analyzing growth and stage transcription level expression profile of the cotton fiber, the fact that the GhNST3 is expressed specifically at secondary wall synthesis stage of the cotton fiber is found, and the GhNST3 is related with synthesis of cell wall is suspected, thereby providing reliable theoretical basis for further study on the effect of the GhNST3 in the aspects of plant growth and development, hormonal regulation, stress resistance and the like.

Description

Technical field [0001] The invention belongs to the field of genetic engineering, and specifically relates to the NST subfamily of cotton NAC transcription factors and their applications. Background technique [0002] In the regulation of plant gene expression, the regulation of transcription factors is a strictly controlled and orderly process. NAC transcription factor is a newly discovered plant-specific transcription factor with various biological functions in the past decade. In 1997, Aida et al. first reported the NAC domain and found that it was encoded in the petunia NAM gene, Arabidopsis ATAFI / 2 and CUC2 genes. The N-terminus of the protein contains a conserved amino acid sequence, named NAC after the first letters of the three genes. Research shows that NAC transcription factors play an important role in plant growth and development, organ establishment, hormone regulation, and defense against various biotic and abiotic stresses. The common feature of this family ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N1/19C12N5/10C12N15/82C12N15/84A01H5/00
Inventor 张根发邴杰刘肖飞
Owner BEIJING NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com