Application of micro ribose nucleic acid (RNA) (miR)-574-5p

A technology for colorectal cancer and therapeutic drugs, applied in the field of biomedical materials small molecule RNA, can solve the problems of low detection rate of early colorectal cancer and limited lesions

Active Publication Date: 2012-04-18
厦门祥安利康生物科技有限公司
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  • Abstract
  • Description
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Problems solved by technology

However, early colorectal cancer lesions are limited to the colonic mucosa and submucosa, and patients often have no clinical symptoms and do not actively seek medical treatment. Therefore, the detection rate of early colorectal cancer is not high at present

Method used

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  • Application of micro ribose nucleic acid (RNA) (miR)-574-5p
  • Application of micro ribose nucleic acid (RNA) (miR)-574-5p
  • Application of micro ribose nucleic acid (RNA) (miR)-574-5p

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Embodiment Construction

[0061] 1. In C57BL / 6-Apc min / + miR-574-5p is negatively correlated with Qki expression in mouse colorectal cancer tissue samples

[0062] Bioinformatics analysis showed that there were many potential binding sites of miRNAs on the 3'UTR of Qki mRNA, including miR-574-5p, miR-717 and miR-20a. Therefore, C57BL / 6-Apc was analyzed using Real-time RT-PCR (qPCR) and western blots experiments min / + Expression levels of Qki and some miRNAs in mouse colorectal cancer tissue samples and normal C57BL / 6 mouse colorectal tissue samples. Compared with normal C57BL / 6 mouse colorectal tissue samples, C57BL / 6-Apc min / + The expression levels of total Qki mRNA / protein (pan-Qki / pan-QKI) and Qki5 / 6 / 7 mRNA / protein were significantly decreased in mouse colorectal cancer tissue samples ( figure 1 with figure 2 ), the expression levels of miR-574-5p, miR-717 and miR-20a were significantly increased, while the expression levels of miR-200b, miR-466g and miR-17 were not significantly changed ( im...

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Abstract

The invention discloses application of micro ribose nucleic acid (RNA) (miR)-574-5p, relates to miR of biomedical materials, and provides application of the miR-574-5p to preparation of a medicine for treating colorectal cancer and a diagnostic reagent for colorectal cancer. The miR-574-5p regulates target genes Qki (quaking) of the miR-574-5p to influence signal transduction of beta-catenin / Wnt and p27, promote proliferation of colorectal cancer cells and inhibit differentiation of the colorectal cancer cells, and promote migration and invasion of the colorectal cancer cells. In the colorectal cancer cells, the expression of the miR-574-5p is extremely high, so that the expression of the miR-574-5p and downstream QKI, beta-catenin and p27 can be detected so as to diagnose tumor, and evaluate the tumor metastasis and prognosis; and in the colorectal cancer cells in which the expression of the miR-574-5p is extremely high, the expression of the miR-574-5p can be inhibited so as to inhibit the occurrence and development of colorectal cancer.

Description

technical field [0001] The invention relates to a biomedical material small molecular RNA, in particular to the use of a small molecular RNA—miR-574-5p in the preparation of colorectal cancer treatment medicine and diagnostic reagent. Background technique [0002] 1) Colorectal cancer [0003] Colorectal cancer is a common malignant tumor occurring in the digestive tract. It is one of the high incidence of human malignant tumors, and its incidence has been on the rise. Among malignant tumors in the world, the incidence of colorectal cancer ranks third, and the mortality rate ranks second. According to statistics, there are about 1 million new patients with colorectal cancer every year in the world. At the same time, about 500,000 people worldwide die from colorectal cancer every year. With my country's economic development and continuous improvement of people's living standards, as well as changes in lifestyle and dietary structure, colorectal cancer has been on the rise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00C12Q1/68G01N33/574G01N33/68G01N33/96A61P35/00
Inventor 杨云青蔡建春纪顺龙张君叶耿泰王霖沛张田甜王涛罗羽郭宗圣
Owner 厦门祥安利康生物科技有限公司
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