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Method for preparing ayfivin compound protein feed additive by degrading feather with bacillus licheniformis

A technology of Bacillus licheniformis and feed additives, applied in the field of resource utilization of waste, to achieve the effects of saving resources, high compound content of bacterial peptides, and simple process

Active Publication Date: 2013-04-24
湖北华扬科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of probiotics has not yet involved the degradation and utilization of discarded feathers

Method used

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  • Method for preparing ayfivin compound protein feed additive by degrading feather with bacillus licheniformis
  • Method for preparing ayfivin compound protein feed additive by degrading feather with bacillus licheniformis
  • Method for preparing ayfivin compound protein feed additive by degrading feather with bacillus licheniformis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1: the pretreatment of feather

[0049] After the original feathers are purchased from the vegetable market, they must first be spread out to remove impurities such as duck skin and viscera; then rinse and soak in tap water for 20 minutes, press the water dry, soak for another 10 minutes, press the water dry, and air-dry them Prepare 0.02% NaOH solution, heat it to boiling, put the feathers into it (about 200g of dry feathers can be processed per liter of the NaOH solution), take out the feathers after 15 minutes, filter the NaOH solution and squeeze the feathers until no solution seeps out, Soak in tap water for 2 minutes, squeeze the feather again until no solution seeps out. until the feather pH is neutral. Dry the feathers at 60°C until the quality does not increase any more.

Embodiment 2

[0050] Embodiment 2: the preparation of seed solution

[0051] Streak the CCTCCM208251 strain preserved in the eggplant-shaped bottle on a solid LB plate to activate it. Select a single colony from the above LB plate and inoculate in 12 bottles of 250 mL Erlenmeyer flasks containing 100 mL of liquid LB medium, put them in a shaker, and cultivate them at 37 ° C and 220 rpm for 10 h. Samples were taken from each bottle of fermentation broth and sent for microscopic examination. All 12 bottles of fermentation broth were free of pollution and grew vigorously. Inoculate above-mentioned primary seed fermented liquid in the 40L fermentation tank by the ratio of 10%, contain 25L liquid LB culture medium in the tank, carry out secondary seed expansion cultivation. After 7 hours of fermentation, samples were taken for microscopic examination, and the results of microscopic examination showed no pollution. Keep the uncontaminated fermentation broth in the fermenter for later use. Micr...

Embodiment 3

[0052] Embodiment 3: the shake flask fermentation of inoculating 100mL feather fermentation substrate by fermentation substrate volume 5%:

[0053] Feather bio-fermentative degradation was carried out in 250mL Erlenmeyer flasks, and the filling volume of each Erlenmeyer flask was 100mL. Inoculate Bacillus licheniformis at 5% in the fermentation substrate sterilized at 121°C for 30 minutes. The composition of the fermentation substrate is: (w / v%) 0.3% potassium nitrate, 0.1% dipotassium hydrogen phosphate, 0.06% dihydrogen phosphate Potassium, Magnesium Chloride Hexahydrate 0.04%, NaCl 0.5%, Sorbitol 0.1%, Feather 2%. It was fermented on a shaking table at 37° C. and 200 rpm, and the fermentation broth was observed every 12 hours until the feathers in the fermentation broth no longer decreased to stop the fermentation. The sediment contained a large amount of probiotic Bacillus licheniformis. Sampling was sent for inspection of the bacteria content and feather degradation rate...

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Abstract

The invention provides a method for preparing an ayfivin compound protein feed additive by degrading feather by using the characteristics that bacillus licheniformis CCTCCM208251 as a probiotics has an activity of degrading feather and can grow with the feather as a unique carbon source and a unique nitrogen source. The method comprises the following steps of: treating the feather, preparing a seed solution, preparing a substrate, fermenting the substrate, filtering, spraying and drying, and the like. The method has the characteristics of simple process, waste recycling, environment friendliness, resource saving and high ayfivin compound content, and accords with the feed safety. The feather degradation rate can reach 80 percent. The method can be used for effectively treating the waste feature and obtaining a large quantity of soluble protein small peptide and the probiotics; and ayfivin compound protein is used for feed adding, and has better economic benefit and social benefit.

Description

technical field [0001] The invention belongs to the field of resource utilization of waste, and in particular relates to a Bacillus licheniformis strain with feather-degrading activity and a method for preparing feed containing probiotic protein by degrading feathers. Background technique [0002] Feathers are derivatives of keratinized epidermal cells of birds and poultry, in which the protein content is as high as 90%. The mass consumption of animals produces a lot of feather waste, and the traditional treatment methods are mainly landfill and incineration, resulting in a waste of feather protein resources. The main protein in feathers is keratin, which is cross-linked into a complex three-dimensional network structure due to a large number of disulfide bonds. It has good stability and insolubility, and it is difficult to hydrolyze it by general methods. The current theoretical degradation pathways include physical degradation, chemical degradation, enzymatic degradation ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/10
Inventor 张桂敏龚一轩
Owner 湖北华扬科技发展有限公司
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