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Kit for detecting echinococcus granulosus pathogen from dog excrement

A technology of Echinococcus granulosus and a kit is applied in the field of detection kits for detecting the presence of Echinococcus granulosus pathogens in dog feces, and can solve the problems of low sensitivity and specificity, infecting people and the surrounding environment danger, and the like, To achieve the effect of strong specificity, high sensitivity and simple operation

Inactive Publication Date: 2012-02-29
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods have certain shortcomings, such as low sensitivity and specificity, and the routine detection method also has the risk of infecting people and the surrounding environment.

Method used

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  • Kit for detecting echinococcus granulosus pathogen from dog excrement
  • Kit for detecting echinococcus granulosus pathogen from dog excrement
  • Kit for detecting echinococcus granulosus pathogen from dog excrement

Examples

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Embodiment Construction

[0013] An example of the present invention is given below.

[0014] In this example, the kit used for detecting Echinococcus granulosus pathogens from dog feces includes: 2×LAMP reaction solution, a mixture composed of four primers, Bst DNA polymerase, standard positive template and chromogenic reagent , the four specific primers are respectively upstream external primer F3: 5'-TGGTTTTAGGTATTTGATTAGGT-3' (SEQ №1); downstream external primer B3: 5'-ACCACTACATACCAACACC-3 (SEQ №2), upstream internal primer FIP: 5 '-TAACCCAAACGTTACCACATCAAAAGAATTCTAGATTTTTGTCTACTATGGGTTGT-3' (SEQ No. 3), downstream internal primer BIP: 5'-ATACTGGTCATTATTTCGGCGGGAATTCCAAACAAAACAATCAACTTCAAC-3' (SEQ No. 4), wherein:

[0015] 2×LAMP reaction solution includes: 40mmol / L Tris-HCl (pH 8.8), 20mmol / L KCl, 16mmol / L MgSO 4 , 20mmol / L (NH 4 ) 2 SO 4 , 0.2% (v / v) Tween 20, 1.6mol / L Betaine and 2.5mmol / L dNTP;

[0016] The primer mixture includes: 40pmol / L upstream internal primer FIP, 40pmol / L downstrea...

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Abstract

The invention discloses a loop-mediated isotheral amplification (LAMP) detection kit for detecting whether echinococcus granulosus pathogen exists in dog excrement or not and application of the kit. The kit comprises four specific primers, wherein the four specific primers comprise an upstream external primer SEQ No. 1, a downstream external primer SEQ No. 2, an upstream internal primer SEQ No. 3 and a downstream internal primer SEQ No. 4. The kit also comprises LAMP reaction liquid, a primer mixture, Bst DNA polymerase, a standard positive template and a color developing agent, so that the kit is convenient to use. The kit can quickly detect whether the echinococcus granulosus pathogen exists in the dog excrement or not, has high sensitivity and high specificity, and is easy and convenient to operate.

Description

technical field [0001] The present invention relates to a detection kit and the application of the kit. Specifically, the present invention relates to a detection kit for detecting whether there is Echinococcus granulosus (Echinococcus granulosus) pathogen in dog feces and the application of the kit. . Background technique [0002] Echinococcus granulosus, the larva of Echinococcus granulosus, can infect a variety of animals (including humans), and can cause zoonotic parasitic diseases that endanger human health and hinder the development of animal husbandry, that is, echinococcosis granulosus, Also known as cystic echinococcosis or cystic echinococcosis. There are currently 10 genotypes of Echinococcus granulosus, namely G1-G10. In my country, there are mainly two types, G1 and G6, of which the G1 type is the most widely distributed and the most harmful. [0003] Canids are the ultimate hosts of Echinococcus granulosus. Adults parasitize in their small intestines, and eg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 贾万忠倪兴维闫鸿斌娄忠子李宏民
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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