Artificial insemination instrument for queen bee
A technology of queen bee and belly hook, which is applied in animal husbandry and other fields, can solve the problems of inconvenient adjustment, achieve the effect of reducing difficulty, simple operation, and overcoming inconvenient adjustment
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Embodiment 1
[0029] refer to figure 1 , figure 1 It is a schematic structural view of the queen bee artificial insemination apparatus in Example 1 of the present invention; as shown in the figure, it includes: a base 1 and an abdominal hook fixing mechanism 2 located thereon, a queen bee fixing mechanism 3, a back hook fixing mechanism 4 and a syringe fixing mechanism Mechanism 5; The queen bee fixing mechanism 3 is positioned at the middle of the abdomen hook fixing mechanism 2 and the back hook fixing mechanism 4, and is used to fix the queen bee; The abdomen hook fixing mechanism 2 and the back hook fixing mechanism 4 are fixed with the abdomen hook and the back hook respectively Not shown), the abdominal hook and the back hook are used to open the sting chamber of the queen bee; the syringe fixing mechanism 5 is used to fix the syringe 57 that is loaded with semen. The queen bee insemination apparatus in this embodiment 1 can realize the fixation of the queen bee, and at the same time...
Embodiment 2
[0038] The present embodiment 2 is based on an application method of the queen bee artificial insemination instrument provided in embodiment 1, and the artificial insemination is carried out by planting the bee farm, which includes the following steps:
[0039] 1) Cultivate drones and queen bees for breeding according to the apiary conservation plan;
[0040] 2) The buffer solution is prepared for use, and the back hook, abdominal hook and vaginal probe (which is an auxiliary tool used in the operation, not shown in the drawings) are sterilized with alcohol, and the syringe needle is boiled and sterilized.
[0041] 3) Semen collection: 12-14 days after the drone emerges out of the room, it can be used for sperm collection. Squeeze with fingers to make the drone ejaculate, and adjust the syringe 57 under a stereomicroscope to get the sperm. After sucking 8-10 microliters of semen with the syringe 57, the queen bee is put into the queen bee fixing mechanism 3 of the queen bee i...
Embodiment 3
[0044] Present embodiment 3 is another application method based on the queen bee artificial insemination instrument provided in embodiment 1, the establishment of inbred line (mother-child backcross), it may further comprise the steps:
[0045] 1) Queen bee and drone preparation: After the queen bee is sexually mature, do not allow it to mate, and treat it with carbon dioxide anesthesia for two consecutive days (10 minutes each time), so that the queen bee produces unfertilized eggs. Buckle the king and wait for the male bee to become sexually mature.
[0046] 2) Semen collection: 12-14 days after the drone emerges out of the room, it can be used for sperm collection. Squeeze with fingers to make the drone ejaculate, and adjust the syringe 57 under a stereomicroscope to get the sperm. After sucking 8-10 microliters of semen with the syringe 57, the queen bee is put into the queen bee fixing mechanism 3 of the queen bee insemination apparatus provided in Example 1, and the car...
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